Transcript Diapositive 1 - Leukemia Net

Multiparametric flow cytometry of
normal human bone marrow:
analysis and display strategies
GEIL-GTLLF
2008
Part three : Monocytic maturation
III.1
CD45
FLn
CD45
Strategy
SSC
SSC
Display
SSC
SSC
SSC
SSC
Backgating
CD45
CD45
CD45
FLy
FLy
FLy
Monober
FLx
FLx
FLx
III.2
CD45
CD45
SSC
SSC
SSC
CD45
CD45
CD45
FL2+
FL1+
FL3
FL3
FL3+
FL2
FLn
Strategy
Display
FL1
SSC
SSC
SSC
Crossgating
FL1
FL2
III.3
Informative combinations
Tube 1 : CD11b/CD14/CD16/CD45
CD11b/CD14
p. III.5
CD11b/CD16
p. III.6
CD14/CD16
p. III.7
Tube 5 : CD38/DR/CD34/CD45
CD38/HLADR
CD38/CD34
p. III.22
HLADR/CD34
Crossgating
Crossgating
p. III-8
p. III.21
p. III.23
p. III.24
Tube 2 : CD11b/CD117/CD34/CD45
CD11b/CD117
p. III-9
CD11b/CD34
p. III-10
CD117/CD34
p. III-11
Crossgating
p. III-12
Tube 7 : DR/CD117/CD34/CD45
HLADR/CD117
HLADR/CD34
CD117/CD34
p. III.27
Crossgating
p. III.28
Tube 3 : CD36/CD71/CD11c/CD45
CD36/CD71
p. III-13
CD36/CD11c
p. III.14
CD71/CD11c
p. III.15
Crossgating
p. III.16
Tube 9 : CD64/CD24/CD10/CD45
CD64/CD24
p. III.29
CD64/CD10
p. III.30
CD24/CD10
p. III.31
Crossgating
p. III.32
p. III.25
p. III.26
Tube 4 : CD34/CD13/CD33/CD45
CD34/CD13
p. III.17
CD34/CD33
CD13/CD33
Crossgating
p. III.18
p. III.19
p. III.20
IIII.4
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45ECD
CD14PE
CD14PE
CD11bFITC
FLx
CD45ECD
CD14PE
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45ECD
SSC
SSC
SSC
CD11b/CD14
CD11bFITC
Gating CD11b
CD11bFITC
Gating CD14
III.5
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45ECD
CD16PC5
CD16PC5
CD11bFITC
FLx
CD45ECD
CD16PC5
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45ECD
SSC
SSC
SSC
CD11b/CD16
CD11bFITC
Gating CD11b
CD11bFITC
Gating CD16
III.6
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45ECD
CD16PC5
CD16PC5
CD14PE
FLx
CD45ECD
CD16PC5
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45ECD
SSC
SSC
SSC
CD14/CD16
CD14PE
Gating CD14
CD14PE
Gating CD16
III.7
CD45ECD
Gating CD 16
SSC
FL2
SSC-Height
CD45ECD
CD16PC5
CD14PE
CD11bFITC
FL1
CD45
FL1+
CD45ECD
CD16PC5
SSC-Height
SSC-Height
FL1
CD45
FL2+
FL3
SSC
CD45
FL3+
FL3
SSC
FL2
Crossgating CD11b/CD14/CD16
CD11bFITC
Gating CD14
CD14PE
Gating CD11b
III.8
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45APC
CD117PE
CD117PE
CD11bFITC
FLx
CD45APC
CD117PE
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45APC
SSC
SSC
SSC
CD11b/CD117
CD11bFITC
Gating CD11b
CD11bFITC
Gating CD117
III.9
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45APC
CD34PC7
CD34PC7
CD11bFITC
FLx
CD45APC
CD34PC7
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45APC
SSC
SSC
SSC
CD11b/CD34
CD11bFITC
Gating CD11b
CD11bFITC
Gating CD34
III.10
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45APC
CD34PC7
CD34PC7
CD117PE
FLx
CD45APC
CD34PC7
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45APC
SSC
SSC
SSC
CD117/CD34
CD117PE
Gating CD117
CD117PE
Gating CD34
III.11
CD45APC
Gating CD 34
SSC
FL2
SSC-Height
CD45APC
CD34PC7
CD117PE
CD11bFITC
FL1
CD45
FL1+
CD45APC
CD34PC7
SSC-Height
SSC-Height
FL1
CD45
FL2+
FL3
SSC
CD45
FL3+
FL3
SSC
FL2
Crossgating CD11b/CD117/CD34
CD11bFITC
Gating CD117
CD117PE
Gating CD11b
III.12
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45ECD
CD71PE
CD71PE
CD36FITC
FLx
CD45ECD
CD71PE
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45ECD
SSC
SSC
SSC
CD36/CD71
CD36FITC
Gating CD36
CD36FITC
Gating CD71
III.13
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45ECD
CD11cPC5
CD11cPC5
CD36FITC
FLx
CD45ECD
CD11cPC5
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45ECD
SSC
SSC
SSC
CD36/CD11c
CD36FITC
Gating CD36
CD36FITC
Gating CD11c
III.14
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45ECD
CD11cPC5
CD11cPC5
CD71PE
FLx
CD45ECD
CD11cPC5
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45ECD
SSC
SSC
SSC
CD71/CD11c
CD71PE
Gating CD71
CD71PE
Gating CD11c
III.15
CD45ECD
Gating CD11c
SSC
FL2
SSC-Height
CD45ECD
CD11cPC5
CD71PE
CD36FITC
FL1
CD45
FL1+
CD45ECD
CD11cPC5
SSC-Height
SSC-Height
FL1
CD45
FL2+
FL3
SSC
CD45
FL3+
FL3
SSC
FL2
Crossgating CD36/CD71/CD11c
CD36FITC
Gating CD71
CD71PE
Gating CD36
III.16
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45PC7
CD13PE
CD13PE
CD34FITC
FLx
CD45PC7
CD13PE
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45PC7
SSC
SSC
SSC
CD34/CD13
CD34FITC
Gating CD34
CD34FITC
Gating CD13
III.17
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45PC7
CD33APC
CD33APC
CD34FITC
FLx
CD45PC7
CD33APC
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45PC7
SSC
SSC
SSC
CD34/CD33
CD34FITC
Gating CD34
CD34FITC
Gating CD33
III.18
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45PC7
CD33APC
CD33APC
CD13PE
FLx
CD45PC7
CD33APC
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45PC7
SSC
SSC
SSC
CD13/CD33
CD13PE
Gating CD13
CD13PE
Gating CD33
III.19
CD45PC7
Gating CD33
SSC
FL2
SSC-Height
CD45PC7
CD33APC
CD13PE
CD34FITC
FL1
CD45
FL1+
CD45PC7
CD33APC
SSC-Height
SSC-Height
FL1
CD45
FL2+
FL3
SSC
CD45
FL3+
FL3
SSC
FL2
Crossgating CD34/CD13/CD33
CD34FITC
Gating CD13
CD13PE
Gating CD34
III.20
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45ECD
HLADRPE
HLADRPE
CD38FITC
FLx
CD45ECD
HLADRPE
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45ECD
SSC
SSC
SSC
CD38/HLADR
CD38FITC
Gating CD38
CD38FITC
Gating HLADR
III.21
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45ECD
CD34PC5
CD34PC5
CD38FITC
FLx
CD45ECD
CD34PC5
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45ECD
SSC
SSC
SSC
CD38/CD34
CD38FITC
Gating CD38
CD38FITC
Gating CD34
III.22
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45ECD
CD34PC5
CD34PC5
HLADRPE
FLx
CD45ECD
CD34PC5
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45ECD
SSC
SSC
SSC
HLADR/CD34
HLADRPE
Gating HLADR
HLADRPE
Gating CD34
III.23
CD38FITC
Gating CD 34
SSC
FL2
SSC-Height
CD45ECD
CD34PC5
HLADRPE
CD45ECD
FL1
CD45
FL1+
CD45ECD
CD34PC5
SSC-Height
SSC-Height
FL1
CD45
FL2+
FL3
SSC
CD45
FL3+
FL3
SSC
FL2
Crossgating CD38/HLADR/CD34
CD38FITC
Gating DR
HLADRPE
Gating CD38
III.24
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45ECD
CD117PE
CD117PE
HLADRFITC
FLx
CD45ECD
CD117PE
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45ECD
SSC
SSC
SSC
HLADR/CD117
HLADRFITC
Gating HLADR
HLADRFITC
Gating CD117
III.25
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45ECD
CD34PC5
CD34PC5
HLADRFITC
FLx
CD45ECD
CD34PC5
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45ECD
SSC
SSC
SSC
HLADR/CD34
HLADRFITC
Gating DR
HLADRFITC
Gating CD34
III.26
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45ECD
CD34PC5
CD34PC5
CD117PE
FLx
CD45ECD
CD34PC5
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45ECD
SSC
SSC
SSC
CD117/CD34
CD117PE
Gating CD117
CD117PE
Gating CD34
III.27
CD45ECD
Gating CD 34
SSC
FL2
SSC-Height
CD45ECD
CD34PC5
CD117PE
HLADRFITC
FL1
CD45
FL1+
CD45ECD
CD34PC5
SSC-Height
SSC-Height
FL1
CD45
FL2+
FL3
SSC
CD45
FL3+
FL3
SSC
FL2
Crossgating HLADR/CD117/CD34
HLADRFITC
Gating CD117
CD117PE
Gating HLADR
III.28
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45PC7
CD24PE
CD24PE
CD64FITC
FLx
CD45PC7
CD24PE
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45PC7
SSC
SSC
SSC
CD64/CD24
CD64FITC
Gating CD64
CD64FITC
Gating CD24
III.29
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45PC7
CD10PC5
CD10PC5
CD64FITC
FLx
CD45PC7
CD10PC5
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45PC7
SSC
SSC
SSC
CD64/CD10
CD64FITC
Gating CD64
CD64FITC
Gating CD10
III.30
FLy
FLy
FLy
Monober
FLx
SSC-Height
CD45PC7
CD10PC5
CD10PC5
CD24PE
FLx
CD45PC7
CD10PC5
SSC-Height
SSC-Height
FLx
CD45
CD45
CD45
CD45PC7
SSC
SSC
SSC
CD24/CD10
CD24PE
Gating CD24
CD24PE
Gating CD10
III.31
CD45PC7
Gating CD10
SSC
FL2
SSC-Height
CD45PC7
CD10PC5
CD24PE
CD64FITC
FL1
CD45
FL1+
CD45PC7
CD10PC5
SSC-Height
SSC-Height
FL1
CD45
FL2+
FL3
SSC
CD45
FL3+
FL3
SSC
FL2
Crossgating CD64/CD24/CD10
CD64FITC
Gating CD24
CD24PE
Gating CD64
III.32