Transcript Diapositive 1 - European Leukemia Net: Network Info

Multiparametric flow cytometry of
normal human bone marrow:
analysis and display strategies
GEIL-GTLLF
2008
Part one : Leukocyte subsets
A colour code is applied trhoughout this atlas:
Granulocytes in red
Monocytes in green
Lymphocytes in purple
All other cells, in a region of maturation defined by the exclusion of mature
cell types and thus dubbed « bermudes » in cyan
I.1
Combinations
Tube 1 : CD11b/CD14/CD16/CD45
CD11b/CD14
p. I-5
CD11b/CD16
p. I-6
CD14/CD16
p. I-7
Tube 7 : HLADR/CD117/CD34/CD45
HLADR/CD117
p. I.29
DR/CD34
p. I.30
CD117/CD34
p. I.31
Tube 2 :CD11b/CD117/CD34/CD45
CD11b/CD117
p. I-9
CD11b/CD34
p. I-10
CD117/CD34
p. I-11
Tube 3 :CD36/CD71/CD11c/CD45
CD36/CD71
p. I-13
CD36/CD11c
p. I-14
Tube 8 : CD34/CD56/CD19/CD45
CD34/CD56
p. I.33
CD34/CD19
p. I.34
CD56/CD19
p. I.35
Tube 9 : CD64/CD24/CD10/CD45
CD64/CD24
p. I.37
CD64/CD10
p. I.38
CD71/CD11c
p. I-15
Tube 4 : CD34/CD13/CD33/CD45
CD34/CD13
p. I-17
CD34/CD33
p. I.18
CD13/CD33
p. I.19
Tube 5 : CD38/HLADR/CD34/CD45
CD24/CD10
p. I.39
Tube 10 : CD7/CD117/CD34/CD45
CD7/CD117
p. I.41
CD7/CD34
p. I.42
CD117/CD34
p. I.43
Tube 11 : CD22/CD10/CD20/CD45
CD38/HLADR
p. I.21
CD38/CD34
p. I.22
HLADR/CD34
p. I.23
Tube 6 : CD38/CD10/CD19/CD45
CD38/CD10
p. I.25
CD38/CD19
p. I.26
CD10/CD19
p. I.27
CD22/CD10
p. I.45
CD22/CD20
p. I.46
CD10/CD20
p. I.47
Tube 12 : CD4/CD2/CD56/CD45
CD4/CD2
p. I.49
CD4/CD56
p. I.50
CD2/CD56
p. I.51
I.2
FLx
FLy
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
Leukocyte subsets display
FLx
FLx
I.3
CD11b/CD14/CD16/CD45
TUBE 1
Granulocytes
Monocytes
Lymphocytes
B
T
Bermudes
NK
CD11b/CD14
x
x
x
x
CD11b/CD16
x
x
x
x
CD14/CD16
x
x
GM
progenitors
B lineage
progenitors
x
I.4
FSC-Height
FLy
FLx
CD14PE
CD45APC
CD14PE
CD14PE
CD11bFITC
FLx
CD11bFITC
CD14PE
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD11b/CD14
CD11bFITC
CD11bFITC
I.5
FSC-Height
FLy
FLx
CD16PC7
CD45APC
CD16PC7
CD16PC7
CD11bFITC
FLx
CD11bFITC
CD16PC7
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD11b/CD16
CD11bFITC
CD11bFITC
I.6
FSC-Height
FLy
FLx
CD16PC7
CD45APC
CD16PC7
CD16PC7
CD14PE
FLx
CD14PE
CD16PC7
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD14/CD16
CD14PE
CD14PE
I.7
CD11b/CD117/CD34/CD45
TUBE 2
Granulocytes
Monocytes
Lymphocytes
B
T
Bermudes
NK
GM
progenitors
CD11b/CD117
x
x
x
x
x
CD11b/CD34
x
x
x
x
x
CD117/CD34
x
x
B lineage
progenitors
x
I.8
FSC-Height
FLy
FLx
SSC-Height
CD45APC
CD117PE
CD117PE
CD11bFITC
FLx
CD45APC
CD117PE
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD11b/CD117
CD11bFITC
CD11bFITC
I.9
FSC-Height
FLy
FLx
CD34PC5
CD45APC
CD34PC5
CD34PC5
CD11bFITC
FLx
CD11bFITC
CD34PC5
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD11b/CD34
CD11bFITC
CD11bFITC
I.10
FSC-Height
FLy
FLx
CD34PC5
CD45APC
CD34PC5
CD34PC5
CD117PE
FLx
CD117PE
CD34PC5
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD117/CD34
CD117PE
CD117PE
I.11
CD36/CD71/CD11c/CD45
TUBE 3
Granulocytes
Monocytes
Lymphocytes
B
T
Bermudes
NK
GM
progenitors
CD 36/CD71
x
x
x
CD36/CD11c
x
x
x
CD71/CD11c
x
x
x
B lineage
progenitors
I.12
FSC-Height
FLy
FLx
CD71PE
CD45APC
CD71PE
CD71PE
CD36FITC
FLx
CD36FITC
CD71PE
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD36/CD71
CD36FITC
CD36FITC
I.13
FSC-Height
FLy
FLx
CD11cPC5
CD45APC
CD11cPC5
CD11cPC5
CD36FITC
FLx
CD36FITC
CD11cPC5
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD36/CD11c
CD36FITC
CD36FITC
I.14
CD71PE
FLy
FLx
CD11cPC5
CD45APC
CD11cPC5
CD11cPC5
FSC-Height
FLx
CD71PE
CD11cPC5
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD71/CD11c
CD71PE
CD71PE
I.15
CD34/CD13/CD33/CD45
TUBE 4
Granulocytes
Monocytes
Lymphocytes
B
T
Bermudes
NK
GM
progenitors
CD34/CD13
x
x
x
CD34/CD33
x
x
x
CD13/CD33
x
x
x
B lineage
progenitors
I.16
FSC-Height
FLy
FLx
CD13PE
CD45PC7
CD13PE
CD13PE
CD34FITC
FLx
CD34FITC
CD13PE
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD34/CD13
CD34FITC
CD34FITC
I.17
FSC-Height
FLy
FLx
CD33APC
CD45PC7
CD33APC
CD33APC
CD34FITC
FLx
CD34FITC
CD33APC
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD34/CD33
CD34FITC
CD34FITC
I.18
FSC-Height
FLy
FLx
CD33APC
CD45PC7
CD33APC
CD33APC
CD13PE
FLx
CD13PE
CD33APC
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD13/CD33
CD13PE
CD13PE
I.19
CD38/HLADR/CD34/CD45
TUBE 5
Granulocytes
Monocytes
Lymphocytes
B
CD38/DR
x
CD38/CD34
x
DR/CD34
x
x
x
T
Bermudes
NK
GM
progenitors
B lineage
progenitors
x
x
x
x
x
I.20
FSC-Height
FLy
FLx
HLADRPE
CD45ECD
HLADRPE
HLADRPE
CD38FITC
FLx
CD38FITC
HLADRPE
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD38/HLADR
CD38FITC
CD38FITC
I.21
FSC-Height
FLy
FLx
CD34PC5
CD45ECD
CD34PC5
CD34PC5
CD38FITC
FLx
CD38FITC
CD34PC5
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD38/CD34
CD38FITC
CD38FITC
I.22
FSC-Height
FLy
FLx
CD34PC5
CD45ECD
CD34PC5
CD34PC5
HLADRPE
FLx
HLADRPE
CD34PC5
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
HLADR/CD34
HLADRPE
HLADRPE
I.23
CD38/CD10/CD19/CD45
TUBE 6
Granulocytes
Monocytes
Lymphocytes
GM
progenitors
B lineage
progenitors
x
x
x
x
x
x
x
x
B
CD38/CD10
x
CD10/CD19
x
x
CD38/CD19
x
Bermudes
T
NK
I.24
FSC-Height
FLy
FLx
CD10PE
CD45Cy7
CD10PE
CD10PE
CD38FITC
FLx
CD38FITC
CD10PE
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD38/CD10
CD38FITC
CD38FITC
I.25
FSC-Height
FLy
FLx
CD19Cy5
CD45Cy7
CD19Cy5
CD19Cy5
CD38FITC
FLx
CD38FITC
CD19Cy5
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD38/CD19
CD38FITC
CD38FITC
I.26
FSC-Height
FLy
FLx
CD19Cy5
CD45Cy7
CD19Cy5
CD19Cy5
CD10PE
FLx
CD10PE
CD19Cy5
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD19/CD10
CD10PE
CD10PE
I.27
HLADR/CD117/CD34/CD45
TUBE 7
Granulocytes
Monocytes
Lymphocytes
B
DR/CD117
x
DR/CD34
x
CD117/CD34
x
x
T
Bermudes
NK
GM
progenitors
x
x
x
B lineage
progenitors
x
x
I.28
FSC-Height
FLy
FLx
CD117PE
CD45Cy7
CD117PE
CD117PE
HLADRFITC
FLx
HLADRFITC
CD117PE
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
HLADR/CD117
HLADRFITC
HLADRFITC
I.29
FSC-Height
FLy
FLx
CD34Cy5
CD45Cy7
CD34Cy5
CD34Cy5
HLADRFITC
FLx
HLADRFITC
CD34Cy5
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
HLADR/CD34
HLADRFITC
HLADRFITC
I.30
FSC-Height
FLy
FLx
CD34Cy5
CD45Cy7
CD34Cy5
CD34Cy5
CD117PE
FLx
CD117PE
CD34Cy5
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD117/CD34
CD117PE
CD117PE
I.31
CD34/CD56/CD19/CD45
TUBE 8
Granulocytes
Monocytes
Lymphocytes
B
CD34/CD56
CD34/CD19
x
CD56/CD19
x
T
Bermudes
NK
GM
progenitors
B lineage
progenitors
x
x
x
x
x
x
x
I.32
FSC-Height
FLy
FLx
CD56PE
CD45APC
CD56PE
CD56PE
CD34FITC
FLx
CD34FITC
CD56PE
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD34/CD56
CD34FITC
CD34FITC
I.33
FSC-Height
FLy
FLx
CD19PC7
CD45APC
CD19PC7
CD19PC7
CD34FITC
FLx
CD34FITC
CD19PC7
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD34/CD19
CD34FITC
CD34FITC
I.34
FSC-Height
FLy
FLx
CD19PC7
CD45APC
CD19PC7
CD19PC7
CD56PE
FLx
CD56PE
CD19PC7
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD56/CD19
CD56PE
CD56PE
I.35
CD64/CD24/CD10/CD45
TUBE 9
Granulocytes
Monocytes
Lymphocytes
B
CD64/CD24
x
x
CD64/CD10
x
x
CD24CD10
x
x
x
T
Bermudes
NK
GM
progenitors
B lineage
progenitors
x
x
x
x
x
x
I.36
FSC-Height
FLy
FLx
CD24PE
CD45Cy7
CD24PE
CD24PE
CD64FITC
FLx
CD64FITC
CD24PE
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD64/CD24
CD64FITC
CD64FITC
I.37
FSC-Height
FLy
FLx
CD10Cy5
CD45Cy7
CD10Cy5
CD10Cy5
CD64FITC
FLx
CD64FITC
CD10Cy5
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD64/CD10
CD64FITC
CD64FITC
I.38
FSC-Height
FLy
FLx
CD10Cy5
CD45Cy7
CD10Cy5
CD10Cy5
CD24PE
FLx
CD24PE
CD10Cy5
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD24/CD10
CD24PE
CD24PE
I.39
CD7/CD117/CD34/CD45
TUBE 10
Granulocytes
Monocytes
Lymphocytes
B
CD7/CD117
x
CD7/CD34
CD117/CD34
x
T
Bermudes
NK
GM
progenitors
x
x
x
x
B lineage
progenitors
x
I.40
FSC-Height
FLy
FLx
CD117PE
CD45Cy7
CD117PE
CD117PE
CD7FITC
FLx
CD7FITC
CD117PE
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD7/CD117
CD7FITC
CD7FITC
I.41
FSC-Height
FLy
FLx
CD34Cy5
CD45Cy7
CD34Cy5
CD34Cy5
CD7FITC
FLx
CD7FITC
CD34Cy5
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD7/CD34
CD7FITC
CD7FITC
I.42
FSC-Height
FLy
FLx
CD34Cy5
CD45Cy7
CD34Cy5
CD34Cy5
CD117PE
FLx
CD117PE
CD34Cy5
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD117/CD34
CD117PE
CD117PE
I.43
CD22/CD10/CD20/CD45
TUBE 11
Granulocytes
Monocytes
Lymphocytes
B
T
Bermudes
NK
GM
progenitors
B lineage
progenitors
CD22/CD10
x
x
CD22/CD20
x
x
CD10/CD20
x
x
I.44
FSC-Height
FLy
FLx
CD10PE
CD45PC7
CD10PE
CD10PE
CD22FITC
FLx
CD22FITC
CD10PE
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD22/CD10
CD22FITC
CD22FITC
I.45
FSC-Height
FLy
FLx
CD20PC5
CD45PC7
CD20PC5
CD20PC5
CD22FITC
FLx
CD22FITC
CD20PC5
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD22/CD20
CD22FITC
CD22FITC
I.46
FSC-Height
FLy
FLx
CD20PC5
CD45PC7
CD20PC5
CD20PC5
CD10PE
FLx
CD10PE
CD20PC5
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD10/CD20
CD10PE
CD10PE
I.47
CD4/CD2/CD56/CD45
TUBE 12
Granulocytes
Monocytes
Lymphocytes
B
T
Bermudes
NK
CD4/CD2
x
x
CD4/CD56
x
x
CD2/CD56
x
x
GM
progenitors
B lineage
progenitors
I.48
FSC-Height
FLy
FLx
CD2PE
CD45APC
CD2PE
CD2PE
CD4FITC
FLx
CD4FITC
CD2PE
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD4/CD2
CD4FITC
CD4FITC
I.49
FSC-Height
FLy
FLx
CD56PC7
CD45APC
CD56PC7
CD56PC7
CD4FITC
FLx
CD4FITC
CD56PC7
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD4/CD56
CD4FITC
CD4FITC
I.50
FSC-Height
FLy
FLx
CD56PC7
CD45APC
CD56PC7
CD56PC7
CD2PE
FLx
CD2PE
CD56PC7
SSC-Height
SSC-Height
FLx
FLx
CD45
FLy
FLy
FSC
FLy
SSC
SSC
CD2/CD56
CD2PE
CD2PE
I.51
Published on ELN website
July 2008
• Contributors
–
–
–
–
–
–
–
–
–
Christine Arnoulet
Marie Christine Béné
Françoise Durrieu
Jean Feuillard
Chantal Fossat
Bernard Husson
Hélène Jouault
Francis Lacombe
Marc Maynadié
• Coordination
– Francis Lacombe & Marie Christine Béné