Transcript Diapositive 1 - Leukemia Net

Multiparametric flow cytometry of
normal human bone marrow:
analysis and display strategies
GEIL-GTLLF
2008
Part four : Lymphocytic maturation
IV.1
CD45
FLn
CD45
Strategy
FSC
SSC
SSC
Display
SSC
SSC
SSC
SSC
Backgating
CD45
CD45
FLx
FLy
FLy
FLy
Lymphober
FLx
FLx
IV.2
Informative combinations
Tube 1 : CD11b/CD14/CD16/CD45
Tube 9 : CD64/CD24/CD10/CD45
CD11b/CD16
CD24/CD10
p. IV.4
p. IV.9
Tube 5 : CD38/DR/CD34/CD45
Tube 10 : CD7/CD117/CD34/CD45
CD38/CD34
CD7/CD117
p. IV.10
CD7/CD34
p. IV.11
p. IV.5
.
Tube 6 : CD38/CD10/CD19/CD45
CD38/CD10
p. IV.6
Tube 8 : CD34/CD56/CD19/CD45
CD34/CD19
p. IV.7
CD56/CD19
p. IV.8
Tube 11 : CD22/CD10/CD20/CD45
CD22/CD10
p. IV.12
CD10/CD20
p. IV.13
IV.3
Lymphober
FLy
FLx
FLx
SSCHeight
CD45ECD
CD16PC6
CD16PC5
CD11bFITC
CD45
CD45ECD
CD16PC5
SSC-Height
SSC-Height
FLx
CD45ECD
CD45
FLy
FLy
FSC
SSC
SSC
SSC
CD11b/CD16
CD11bFITC
Gating CD11b
CD11bFITC
Gating CD16
IV.4
Lymphober
FLy
FLx
FLx
SSCHeight
CD45ECD
CD34PC5
CD34PC5
CD38FITC
CD45
CD45ECD
CD34PC5
SSC-Height
SSC-Height
FLx
CD45ECD
CD45
FLy
FLy
FSC
SSC
SSC
SSC
CD38/CD34
CD38FITC
Gating CD38
CD38ITC
Gating CD34
IV.5
Lymphober
FLy
FLx
FLx
SSCHeight
CD45ECD
CD10PE
CD10PE
CD38FITC
CD45
CD45ECD
CD10PE
SSC-Height
SSC-Height
FLx
CD45ECD
CD45
FLy
FLy
FSC
SSC
SSC
SSC
CD38/CD10
CD38FITC
Gating CD38
CD38FITC
Gating CD10
IV.6
Lymphober
FLy
FLx
FLx
SSCHeight
CD45ECD
CD19PC6
CD19PC5
CD34FITC
CD45
CD45ECD
CD19PC5
SSC-Height
SSC-Height
FLx
CD45ECD
CD45
FLy
FLy
FSC
SSC
SSC
SSC
CD34/CD19
CD34FITC
Gating CD34
CD34FITC
Gating CD19
IV.7
Lymphober
FLy
FLx
FLx
SSCHeight
CD45ECD
CD19PC6
CD19PC5
CD56FITC
CD45
CD45ECD
CD19PC5
SSC-Height
SSC-Height
FLx
CD45ECD
CD45
FLy
FLy
FSC
SSC
SSC
SSC
CD56/CD19
CD56FITC
Gating CD56
CD56ITC
Gating CD19
IV.8
Lymphober
FLy
FLx
FLx
SSCHeight
CD45ECD
CD10PC5
CD10PC5
CD24PE
CD45
CD45ECD
CD10PC5
SSC-Height
SSC-Height
FLx
CD45ECD
CD45
FLy
FLy
FSC
SSC
SSC
SSC
CD24/CD10
CD24PE
Gating CD24
CD24PE
Gating CD10
IV.9
Lymphober
FLy
FLx
FLx
SSCHeight
CD45ECD
CD117PE
CD117PE
CD7FITC
CD45
CD45ECD
CD117PE
SSC-Height
SSC-Height
FLx
CD45ECD
CD45
FLy
FLy
FSC
SSC
SSC
SSC
CD7/CD117
CD7FITC
Gating CD7
CD7FITC
Gating CD117
IV.10
Lymphober
FLy
FLx
FLx
SSCHeight
CD45ECD
CD34PC5
CD34PC5
CD7FITC
CD45
CD45ECD
CD34PC5
SSC-Height
SSC-Height
FLx
CD45ECD
CD45
FLy
FLy
FSC
SSC
SSC
SSC
CD7/CD34
CD7FITC
Gating CD7
CD7FITC
Gating CD34
IV.11
Lymphober
FLy
FLx
FLx
SSCHeight
CD45ECD
CD10PE
CD10PE
CD22FITC
CD45
CD45ECD
CD10PE
SSC-Height
SSC-Height
FLx
CD45ECD
CD45
FLy
FLy
FSC
SSC
SSC
SSC
CD22/CD10
CD22FITC
Gating CD22
CD22FITC
Gating CD10
IV.12
Lymphober
FLy
FLx
FLx
SSCHeight
CD45ECD
CD20PC5
CD20PC5
CD10PE
CD45
CD45ECD
CD20PC5
SSC-Height
SSC-Height
FLx
CD45ECD
CD45
FLy
FLy
FSC
SSC
SSC
SSC
CD10/CD20
CD10PE
Gating CD20
CD10PE
Gating CD10
IV.13