Transcript Diapositive 1 - Leukemia Net

Multiparametric flow cytometry of
normal human bone marrow:
analysis and display strategies
GEIL-GTLLF
2008
Part two : Granulocytic maturation
II.1
CD45
Display
FLn
CD45
SSC
Strategy
SSC
SSC
SSC
SSC
SSC
Backgating
CD45
CD45
CD45
FLy
FLy
FLy
Granulober
FLx
FLx
FLx
II.2
CD45
Display
SSC
SSC
SSC
Crossgating
FLn+
FLn
CD45
SSC
SSC
SSC
Strategy
CD45
CD45
CD45
Granulober
FL2+
FL1
FL1+
FL3
FL2
FL3
FL3+
FL1
FL2
II.3
Informative combinations
Tube 1 : CD11b/CD14/CD16/CD45
Tube 5 : CD38/DR/CD34/CD45
CD11b/CD14
p. II-5
CD38/HLADR
p. II.20
CD11b/CD16
p. II-6
CD38/CD34
p. II.21
Crossgating
p. II-7
Crossgating
p. II.22
Tube 2 : CD11b/CD117/CD34/CD45
Tube 7 : DR/CD117/CD34/CD45
CD11b/CD117
p. II-8
HLADR/CD117
p. II.23
CD11b/CD34
p. II-9
HLADR/CD34
p. II.24
CD117/CD34
p. II-10
CD117/CD34
p. II.25
Crossgating
p. II-11
Crossgating
p. II.26
Tube 3 : CD36/CD71/CD11c/CD45
Tube 9 : CD64/CD24/CD10/CD45
CD36/CD71
p. II-12
CD64/CD24
p. II.27
CD36/CD11c
p. II-13
CD64/CD10
p. II.28
CD71/CD11c
p. II-14
CD24/CD10
p.II29
Crossgating
p. II-15
Crossgating
p. II.30
Tube 4 : CD34/CD13/CD33/CD45
CD34/CD13
p. II-16
CD34/CD33
p. II.17
CD13/CD33
Crossgating
p. II.18
p. II.19
II.4
CD45
Granulober
FLx
SSC-Height
CD45ECD
CD14PE
CD14PE
CD11bFITC
FLx
CD45ECD
CD14PE
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45ECD
SSC
SSC
SSC
CD11b/CD14
CD11bFITC
Gating CD11b
CD11bFITC
Gating CD14
II.5
CD45
Granulober
FLx
SSC-Height
CD45ECD
CD16PE
CD16PE
CD11bFITC
FLx
CD45ECD
CD16PE
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45ECD
SSC
SSC
SSC
CD11b/CD16
CD11bFITC
Gating CD11b
CD11bFITC
Gating CD16
II.6
CD45ECD
Gating CD16
SSC
FL2
SSC-Height
CD45ECD
CD16PC5
CD14PE
CD11bFITC
FL1
CD45
FL1+
CD45ECD
CD16PC5
SSC-Height
SSC-Height
FL1
CD45
FL2+
FL3
SSC
CD45
FL3+
FL3
SSC
FL2
Crossgating CD11b/CD14/CD16
CD11bFITC
Gating CD14
CD14PE
Gating CD11b
II.7
CD45
Granulober
FLx
SSC-Height
CD45ECD
CD117PE
CD117PE
CD11bFITC
FLx
CD45ECD
CD117PE
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45ECD
SSC
SSC
SSC
CD11b/CD117
CD11bFITC
Gating CD11b
CD11bFITC
Gating CD117
II.8
CD45
Granulober
FLx
SSC-Height
CD45ECD
CD34PC7
CD34PC7
CD11bFITC
FLx
CD45ECD
CD34PC7
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45ECD
SSC
SSC
SSC
CD11b/CD34
CD11bFITC
Gating CD11b
CD11bFITC
Gating CD34
II.9
CD45
Granulober
FLx
SSC-Height
CD45ECD
CD34PC7
CD34PC7
CD117PE
FLx
CD45ECD
CD34PC7
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45ECD
SSC
SSC
SSC
CD117/CD34
CD117PE
Gating CD117
CD117PE
Gating CD34
II.10
CD45APC
Gating CD34
SSC
FL2
SSC-Height
CD45APC
CD34PC7
CD117PE
CD11bFITC
FL1
CD45
FL1+
CD45APC
CD34PC7
SSC-Height
SSC-Height
FL1
CD45
FL2+
FL3
SSC
CD45
FL3+
FL3
SSC
FL2
Crossgating CD11b/CD117/CD34
CD11bFITC
Gating CD117
CD117PE
Gating CD11b
II.11
CD45
Granulober
FLx
SSC-Height
CD45ECD
CD71PE
CD71PE
CD36FITC
FLx
CD45ECD
CD71PE
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45ECD
SSC
SSC
SSC
CD36/CD71
CD36FITC
Gating CD36
CD36FITC
Gating CD71
II.12
CD45
Granulober
FLx
SSC-Height
CD45ECD
CD11cPC5
CD11cPC5
CD36FITC
FLx
CD45ECD
CD11cPC5
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45ECD
SSC
SSC
SSC
CD36/CD11c
CD36FITC
Gating CD36
CD36FITC
Gating CD11c
II.13
CD45
Granulober
FLx
SSC-Height
CD45ECD
CD11cPC5
CD11cPC5
CD71PE
FLx
CD45ECD
CD11cPC5
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45ECD
SSC
SSC
SSC
CD71/CD11c
CD71PE
Gating CD71
CD71PE
Gating CD11c
II.14
CD45ECD
Gating CD11c
SSC
FL2
SSC-Height
CD45ECD
CD11cPC5
CD71PE
CD36FITC
FL1
CD45
FL1+
CD45ECD
CD11cPC5
SSC-Height
SSC-Height
FL1
CD45
FL2+
FL3
SSC
CD45
FL3+
FL3
SSC
FL2
Crossgating CD36/CD71/CD11c
CD36FITC
Gating CD71
CD71PE
Gating CD36
II.15
CD45
Granulober
FLx
SSC-Height
CD45ECD
CD13PE
CD13PE
CD34FITC
FLx
CD45ECD
CD13PE
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45ECD
SSC
SSC
SSC
CD34/CD13
CD34FITC
Gating CD34
CD34FITC
Gating CD13
II.16
CD45
Granulober
FLx
SSC-Height
CD45ECD
CD33PC5
CD33PC5
CD34FITC
FLx
CD45ECD
CD33PC5
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45ECD
SSC
SSC
SSC
CD34/CD33
CD34FITC
Gating CD34
CD34FITC
Gating CD33
II.17
CD45
Granulober
FLx
SSC-Height
CD45ECD
CD33PC5
CD33PC5
CD13PE
FLx
CD45ECD
CD33PC5
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45ECD
SSC
SSC
SSC
CD13/CD33
CD13PE
Gating CD13
CD13PE
Gating CD33
II.18
CD45ECD
Gating CD33
SSC
FL2
SSC-Height
CD45ECD
CD33PC5
CD13PE
CD34FITC
FL1
CD45
FL1+
CD45ECD
CD33PC5
SSC-Height
SSC-Height
FL1
CD45
FL2+
FL3
SSC
CD45
FL3+
FL3
SSC
FL2
Crossgating CD34/CD13/CD33
CD34FITC
Gating CD13
CD13PE
Gating CD34
II.19
CD45
Granulober
FLx
SSC-Height
CD45ECD
HLADRPE
HLADRPE
CD38FITC
FLx
CD45ECD
HLADRPE
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45ECD
SSC
SSC
SSC
CD38/HLADR
CD38FITC
Gating CD38
CD38FITC
Gating HLADR
II.20
CD45
Granulober
FLx
SSC-Height
CD45ECD
CD34PC5
CD34PC5
CD38FITC
FLx
CD45ECD
CD34PC5
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45ECD
SSC
SSC
SSC
CD38/CD34
CD38FITC
Gating CD38
CD38FITC
Gating CD34
II.21
CD38FITC
Gating CD34
SSC
FL2
SSC-Height
CD45ECD
CD34PC5
HLADRPE
CD45ECD
FL1
CD45
FL1+
CD45ECD
CD34PC5
SSC-Height
SSC-Height
FL1
CD45
FL2+
FL3
SSC
CD45
FL3+
FL3
SSC
FL2
Crossgating CD38/HLADR/CD34
CD38FITC
Gating HLADR
HLADRPE
Gating CD38
II.22
CD45
Granulober
FLx
SSC-Height
CD45ECD
CD117PE
CD117PE
HLADRFITC
FLx
CD45ECD
CD117PE
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45ECD
SSC
SSC
SSC
HLADR/CD117
HLADRFITC
Gating HLADR
HLADRFITC
Gating CD117
II.23
CD45
Granulober
FLx
SSC-Height
CD45ECD
CD34PC5
CD34PC5
HLADRFITC
FLx
CD45ECD
CD34PC5
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45ECD
SSC
SSC
SSC
HLADR/CD34
HLADRFITC
Gating HLADR
HLADRFITC
Gating CD34
II.24
CD45
Granulober
FLx
SSC-Height
CD45ECD
CD34PC5
CD34PC5
CD117PE
FLx
CD45ECD
CD34PC5
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45ECD
SSC
SSC
SSC
CD117/CD34
CD117PE
Gating 117
CD117PE
Gating CD34
II.25
FL1
Gating CD34
Gating CD117
SSC
CD45
FL2+
FL3
SSC
CD45
FL3+
FL3
SSC
FL2
Crossgating HLADR/CD117/CD34
FL1
CD45
FL1+
FL2
Gating HLADR
II.26
CD45
Granulober
FLx
SSC-Height
CD45PC7
CD24PE
CD24PE
CD64FITC
FLx
CD45PC7
CD24PE
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45PC7
SSC
SSC
SSC
CD64/CD24
CD64FITC
Gating CD64
CD64FITC
Gating CD24
II.27
CD45
Granulober
FLx
SSC-Height
CD45PC7
CD10PC5
CD10PC5
CD64FITC
FLx
CD45PC7
CD10PC5
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45PC7
SSC
SSC
SSC
CD64/CD10
CD64FITC
Gating CD64
CD64FITC
Gating CD10
II.28
CD45
Granulober
FLx
SSC-Height
CD45PC7
CD10PC5
CD10PC5
CD24PE
FLx
CD45PC7
CD10PC5
SSC-Height
SSC-Height
FLx
CD45
FLy
FLy
FLy
CD45
CD45PC7
SSC
SSC
SSC
CD24/CD10
CD24PE
Gating CD24
CD24PE
Gating CD10
II.29
CD45PC7
Gating CD10
SSC
FL2
SSC-Height
CD45PC7
CD10PC5
CD24PE
CD64FITC
FL1
CD45
FL1+
CD45PC7
CD10PC5
SSC-Height
SSC-Height
FL1
CD45
FL2+
FL3
SSC
CD45
FL3+
FL3
SSC
FL2
Crossgating CD64/CD24/CD10
CD64FITC
Gating CD24
CD24PE
Gating CD64
II.30