Transcript Malignant Nephroblastoma in a Horse

Elizabeth M. Whitley, DVM, PhD, Dipl. ACVP1
Isabel Hurtado Rodriguez, DVM, MS, Dipl. ACVIM2
Amelia S. Munsterman, DVM, MS, Dipl. ACVS2
1Department
of Pathobiology
2Department of Clinical Sciences
College of Veterinary Medicine
Auburn University
Presented at SEVPAC 2008 – Permission grantedwww.sogonow.com/archives/2007/01/07-week/
for use on SEVPAC website only
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5-year-old, male castrated, American quarter
horse
Used for roping
 Recent, intermittent reluctance to work on the
right hand
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Recent, unexplained weight loss
Presented
at SEVPAC 2008 – Permission granted for use on SEVPAC website only
www.sogonow.com/archives/2007/01/07-week/
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Presented for colic that was non-responsive
to Banamine and sedation
Clinical signs:
 Dehydration, tachycardia, tachypnea
 Copious nasogastric reflux when tubed
 Distended small intestine on ultrasound and
palpation
 Diagnosed a renal mass on ultrasound
Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only
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Rare neoplasm in animals other than pigs or
chickens
Usually observed in young animals
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“Wilms’ tumor” in children
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http://www.nlm.nih.gov/medlineplus/ency/imagepages/90
Presented at SEVPAC 2008 – Permission
granted for use on SEVPAC website only
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Three different morphologies
 Blastemal (undifferentiated, embryonic) cells
 Epithelial cells
 Mesenchymal cells
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Benign or malignant
Presented
at SEVPAC 2008 – Permission granted for use on SEVPAC website only
en.wikibooks.org/.../Pregnancy_and_birth
Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only
Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only
Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only
Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only
Vimentin
Desmin
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Smooth muscle actin
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Cytokeratin
Cytokeratin
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Basement
membrane
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Malignant nephroblastoma, triphasic, kidney,
with metastasis to liver, spleen, and
mesentery.
Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only
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Inactivation of the Wilms’ tumor gene (WT1)
 WT1 protein is a transcription factor that
functions as a tumor suppressor gene
▪ Is expressed during renal and gonadal development
 Defects in WT1 gene prevents normal
differentiation of metanephric stem cells
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Other genetic defects identified in children
with Wilms’ tumor ( including WT2, WT3,
WT4)
Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only
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Thanks to Dr. Eva Sartin, Beth Landreth, Uschi Wolfert for technical assistance in
histopathology.
Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only
Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only
Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only