04 Artemia salina Hatching Lab Analysis Notes

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Transcript 04 Artemia salina Hatching Lab Analysis Notes

With all thine offerings thou shalt offer saltMoses (in Leviticus)
Why salt?
Biological Need For Salt
Salt Sources
Gluphisia septentrionis
Puddling
Why?
Gluphisia, Salt & Reproduction
Sampling Protocol
• Using dropper pipette, extract 1mL
from petri dish
• Place 1mL sample into petri dish lid
• Place lid and sample on top of graph
paper and view under stereoscope
• Count hatched naupili
• Multiply total number of naupili from
sample by 30
• Repeat 3x for each culture/petri dish
• Average results for each culture and
post results.
• Use class averages to support/refute
conclusions
• Clean out dishes, remove labels,
place in wash bin (center island)
Lab Report
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Write in lab notebook
Title
Purpose w/general methodology
Hypothesis w/rationale (reasoning)
Data table (class avg), graph and qualitative
observations
• Conclusion
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Restate hypothesis
Supported, refuted or inconclusive
Selected data citations and elaboration
What does this suggest about the relationship of the
independent variable and dependent?
– Were there any sources of error/confounding variables and how
might they have influenced your conclusion?