Transcript Overview

(Power)Point me in The right
direction in Lab
Mark Walvoord
University of Oklahoma
ABLE 2008
Chicken Wing Microbiology: A
lesson in food safety and
microbioogical technique
• Mariëlle Hoefnagels
• Mark Walvoord
ABLE 2005 Proceedings, Vol. 27:185-200
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•
Two main objectives:
1.
Measure populations of bacteria on chicken wings
2.
Design an experiment to test a treatment that might get rid of the bacteria
Important lessons:
•
Basic microbiology techniques: serial dilution, plate count, aseptic technique
•
Food safety
•
Experimental design (investigative)
Aseptic Technique: Overall goal: Avoid contaminating your solutions or anything sterile, including pipets,
saline, and agar.
For example: Wash your hands before you begin (& afterwards); Wipe down bench with Lysol
before you begin; Discard any plates with existing colonies; Open containers/petri dishes only when
necessary, and then only briefly; Never touch pipet tip to any surface except the solution you are
pipetting; Use each pipet only once, then set aside
Timeline
•Week 1: set up initial counts of bacteria on chicken wings
•Week 2: count bacteria from week 1; design and implement independent experiments
•Week 3: collect data from independent experiments; write report to Wing Nut Chicken Wing Co.
Procedures
Label your plates & test tubes
Make “wing washes” for two chicken wings (per group)
Make serial dilution (through 10-7 in test tubes) for each wing
Inoculate agar plates (2 duplicate plates per tube)
Clean UP
•After, put contaminated trash (gloves, chicken, used pipets) in autoclave bags
•Put “clean” trash (pipet wrappers, paper towels, etc.) in room’s trashcan
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