Transcript Slide 1

Department of Chemistry
Seminar Announcement
Date/Time/Venue
Title/Speaker
19 May (Thu)
11am – 12nn
Label Free Optical Imaging for Biology
and Medicine
@ LT31
Faculty of Science
Blk S16, Level 3
Prof Sunney Xiaoliang Xie
Harvard University, USA
Host : Assoc Prof Xu Qing-Hua
About the Speaker
Prof Sunney X. Xie is a Mallinckrodt Professor of Chemistry and
Chemical Biology at Harvard University. He received his BSc in
chemistry from Peking University in 1984 and PhD in chemistry
from University of California – San Diego under the direction of
Professor John D Simon. He pursued his postdoctoral research
with Professor Graham R Fleming at University of Chicago from
1990 – 1992. His research interests include single molecule
spectroscopy, single molecule enzymology, molecular interactions,
conformational dynamics, and underlying mechanishms of macromolecule
machines, gene expression in living cells and nonlinear optical imaging and
biomedical applications.
Prof Sunney Xie was awarded the Berthold Leibinger Zukunftspreis for Laser
Technology in 2008 and E.O. Lawrence Award in Chemistry in 2009. He is
currently an Honorary Fellow of the Chinese Chemical Society and a member of
National Academy of Science, USA.
Abstract
We recently developed a 3D multiphoton imaging technique, stimulated Raman
scattering (SRS) microscopy, which allows imaging based on vibrational
spectroscopy without the need for staining or fluorescent labeling. Chemical
specificity of the technique is achieved by probing intrinsic vibrational properties of
the molecules. Superb detection sensitivity is achieved by implementation of a
high-frequency detection scheme and surpasses that of other label-free imaging
techniques, such as spontaneous Raman scattering and coherent anti-Stokes
Raman scattering (CARS) microscopy. Compared to CARS, SRS has the advantage
that it does not suffer from an unwanted nonresonant background signal that
distorts vibrational spectra and is linear in analyte concentration. We demonstrate
video-rate SRS imaging in live anesthetized mice and present applications in drug
delivery, lipid metabolism and medical diagnostics of brain pathologies.
All are Welcome