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When things get rough you need new stuff William Lipps Analytical & Measuring Instrument Division July, 2015

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It’s out there

All methods begin with an idea

Are you ready?

How will you detect it, and are your methods fast enough?

MALDI-TOF Mass Spectrometry can identify sub-species in under 2 minutes

Red lines indicating a spectrum match

Algae is toxic and it Kills

LCMSMS Analysis of Microcystins

Polar pesticides and herbicides are difficult by GC

Analyze pesticides and herbicides by LCMSMS, with very little sample prep

Analyze pesticides and herbicides by LCMSMS, with very little sample prep

We can analyze hundreds more pesticides with high selectivity and sensitivity by GCMSMS

Methods can retain full scan capability and add MRM for greater selectivity and sensitivity

(x1,000,000) 4.0

3.0

2.0

Scan Chromatogram

1.0

11.0

11.5

100 % 75 50 25 0 0 12.0

12.5

13.0

13.5

14.0

97 59 50 65 223 162 100 117 135 150 173 203 200 Qualification 251 259 279 250 305 300 341 365 397 350 400

MRM Chromatogram

14.5

15.0

15.5

16.0

16.5

17.0

(x10,000) 1.00

279.00>222.90

279.00>204.90

0.75

0.50

0.25

13.50

13.75

14.00

14.25

Quantification 17.5

Analysis of solid waste for metals requires acid digestion and instrumental analysis,

Or,

And create new methods that use XRF

Primary X-rays Analysis sample Al Cr Pb Cd Vertical axis: X-ray intensity (cps/uA) = Concentration (ppm) – cps/uA – cps/uA – cps/uA – cps/uA Fluorescent X-rays Al Cr Pb X-ray tube Cd Al Cr Cd Pb Detector 1.487

keV 5.412

keV 10.552

keV 23.110

kev Horizontal axis: Energy (keV) = Type of element

An example of a whole rock quantitative analysis of cement

Na-S K-Zn Sr

XRF CRM

Peak Profile of Cement Standard Substance

% MgO 1.75

1.93

% Al 2 O 3 3.95

3.88

% SO 3 21.9

22.4

% CaO 69.6

67.9

Sample Appearance (Press-formed at 250 kN for 30 sec)

Just place liquids, slurry samples, and emulsions in a sample cell Sample cell, film, and 5 mL oil

Elements in Waste Oil (0, 10, 30, and 50 ppm of each element) Blank Sample Overlaid Profiles of Heavy Elements in Waste Oil 10 ppm 30 ppm 50 ppm

Foreign Matter Adhering to a Plastic Extruded Part

Sample Appearance Red circle: Foreign matter Blue circle: Normal position Foreign matter Normal position Overlaid Profiles of Foreign Matter (Red) and Normal Position (Blue)

Let’s reduce toxic chemicals

Replace toxic cadmium with reductase for NOx Sample # Commercial Laboratory Results, Analysis Method EPA 335.2 (mg NO 3 +NO 2 -N/L) Analysis by Reductase (mg NO 3 +NO 2 -N/L) 1 2 3 4 5 6 7 0.8

<0.1

<0.1

0.66

11.8

0.78

2.4

0.94

0.05

0.24

0.68

11.6

0.79

3.11

Should we continue with the Kjeldahl Method for TKN?

Or use HTCO methods and obtain near equal results with TKN

Technology is advancing faster than the methods

It’s time for some new stuff

MALDI TOF – New, faster way to confirm bacteria

LCMSMS – Emerging Contaminants not visible before

GCMSMS – more sensitive, higher throughput

XRF – no digestions, higher throughput

NOx – safer, non toxic reagents

TN – safer and higher throughput than TKN

Thank You, for more information contact me

[email protected]