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Figure S1, FGFR2 copy gain and overexpression in H716 cells A NCI H716 colon adenocarcinoma View Key FGFR2 (MB 122) B KATOIII gastric cancer FGFR2 (MB 122) C Figure S1C: Selective FGFR2 overexpression in H716, KATOIII and SNU16 cell lines in Wooster Cell Line Reporter: KatoIII SNU16 H716 18,823 measured genes 10-123287897 338 samples Figure S2: FGF2 does not further activate FGFR2 in H716 or colon cancer cell lines. SW620 - SW48 + + RKO SW480 - - + H747 DLD1 + - + LS411N - - + - + - + - FGF2 H508 + H716 FGFR2 -150 p-FGFR2 -150 Actin - 40 80-100%activity : Kinase % Control FGFR1 FGFR3 FGFR2 DDR1 MKNK1 FLT4 PIK3CB CSF1R NEK4 NEK11 FGFR4 STK25 STK24 CHUK NUAK1 MAPK13 MAP2K4 NTRK2 MATK ERBB3 ABL1 RIOK2 AKT1 BTK DCLK1 CDK4 PIK3CA HIPK2 EGFR MAP2K6 RPS6KA4 NUAK2 FLT3 HUNK MAPK9 STK3 KDR DYRK2 LCK MAP3K3 TAOK3 TRPM6 CDKL3 EIF2AK2 TSSK1B MAP3K2 PDGFRB PLK1 1.4 1.6 2.4 18 34 35 35 36 54 55 56 57 58 62 67 67 68 68 69 69 70 71 72 72 72 73 73 74 76 76 76 76 77 77 77 77 77 78 78 78 78 78 79 79 79 80 80 80 AAK1 ABL1 ABL2 ACVR1 ACVR1B ACVR2A ACVR2B ACVRL1 ADCK4 AKT2 AKT3 ALK ANKK1 AURKA AURKB AURKC AXL BLK BMP2K BMPR1A BMPR1B BMPR2 BMX BRAF BRSK1 BRSK2 CABC1 CAMK1 CAMK1D CAMK1G CAMK2A CAMK2B CAMK2D CAMK2G CAMK4 CAMKK1 CAMKK2 CASK CDC2L1 CDC2L2 CDC2L5 CDC2L6 CDC42BPA CDC42BPB CDC42BPG Figure S3: AMBIT panel with 100nM PD173074 CDK2 CDK3 CDK4 CDK5 CDK7 CDK8 CDK9 CDKL1 CDKL2 CDKL5 CHEK1 CHEK2 CIT CLK1 CLK2 CLK3 CLK4 CSK CSNK1A1 CSNK1A1L CSNK1D CSNK1E CSNK1G1 CSNK1G2 CSNK1G3 CSNK2A1 CSNK2A2 DAPK1 DAPK2 DAPK3 DCLK2 DCLK3 DDR2 DMPK DSTKY DYRK1A DYRK1B EGFR EIF2AK1 EIF2AK4 EPHA1 EPHA2 EPHA3 EPHA4 EPHA5 EPHA6 EPHA7 EPHA8 EPHB1 EPHB2 EPHB3 EPHB4 EPHB6 ERBB2 ERBB4 ERN1 FER FES FGR FLT1 FLT3 FRAP1 FRK FYN GAK GRK1 GRK4 GRK7 GSK3A GSK3B HCK HIPK1 HIPK3 HIPK4 ICK IGF1R IKBKB IKBKE INSR INSRR IRAK1 IRAK3 IRAK4 ITK JAK1 JAK1 JAK2 JAK3 KIAA0999 KIT LATS1 LATS2 LIMK1 LIMK2 LRRK2 LRRK2 LTK LYN MAK MAL13P1.279 MAP2K1 MAP2K2 MAP2K3 MAP2K5 MAP2K7 MAP3K1 MAP3K10 MAP3K11 MAP3K12 MAP3K13 MAP3K15 MAP3K2 MAP3K4 MAP3K5 MAP3K6 MAP3K7 MAP3K9 MAP4K1 MAP4K2 MAP4K3 MAP4K4 MAP4K5 MAPK1 MAPK10 MAPK11 MAPK12 MAPK14 MAPK15 MAPK3 MAPK4 MAPK6 MAPK7 MAPK8 MAPKAPK2 MAPKAPK5 MARK1 MARK2 MARK3 MARK4 MAST1 MELK MERTK MET MGC42105 MINK1 MKNK2 MST1R MST4 MUSK MYLK MYLK2 MYLK3 MYLK4 MYO3A MYO3B NEK1 NEK2 NEK3 NEK5 NEK6 NEK7 NEK9 NLK NTRK1 NTRK3 OXSR1 PAK1 PAK2 PAK3 PAK4 PAK6 PAK7 PCTK1 PCTK2 PCTK3 PDGFRA PDGFRB PDPK1 PFB0815w PFTK1 PFTK2 PHKG1 PHKG2 PI4KB PIK3C2B PIK3C2G PIK3CA PIK3CD PIK3CG PIM1 PIM2 PIM3 PIP4K2B PIP4K2C PIP5K1A PIP5K1C PKMYT1 PKN1 PKN2 pknB PLK1 PLK2 PLK3 PLK4 PRKAA1 PRKAA2 PRKACA PRKACB PRKCD PRKCE PRKCH PRKCI PRKCQ PRKD1 PRKD2 PRKD3 PRKG1 PRKG2 PRKX PRPF4B PTK2 PTK2B PTK6 RAF1 RET RIOK1 RIOK3 RIPK1 RIPK2 RIPK4 ROCK1 ROCK2 ROS1 RPS6KA1 RPS6KA1 RPS6KA2 RPS6KA2 RPS6KA3 RPS6KA4 RPS6KA5 RPS6KA5 RPS6KA6 RPS6KA6 RPS6KB1 SBK1 SgK110 SGK3 SIK1 SIK2 SLK SNRK SRC SRMS SRPK1 SRPK2 SRPK3 STK10 STK11 STK16 STK17A STK17B STK32A STK32B STK32C STK33 STK35 STK36 STK38 STK38L STK39 STK4 SYK TAOK1 TAOK2 TBK1 TEC TEK TESK1 TGFBR1 TGFBR2 TIE1 TLK1 TLK2 TNIK TNK1 TNK2 TNNI3K TTK TXK TYK2 TYK2 TYRO3 ULK1 ULK2 ULK3 VRK2 WEE1 WEE2 YES1 YSK4 ZAK ZAP70 Figure S4: PD173074 Inhibits pFGFR2 and selectively inhibits NCI-H716 growth B A IC50 100 80 60 40 20 0 T = 3 days Figure S5: pRSK S359/363 (ERK phosphorylation site) is inhibited by PD173074 PD173074 : P-359/363 RSK + -100 -75 GAPDH Figure S6: L-547 and Rapamycin inhibit Akt and S6RP phosphorylation B A 1uM L547 pAkt Akt : - + Rapamycin -60 pS6RP + -35 -60 S6RP GAPDH - -35 -35 -35 GAPDH Figure S7: PD173074 causes cell death in NCI-H716 cells . Untreated PD173074 100nM Figure S8: E cadherin and EPCAM are not expressed in H716 cells SW620 - SW48 + + - RKO SW480 - + H747 DLD1 + - + LS411N - - + - + - + - H80 H508 + H716 FGF2 -150 CDH1 -- 120 EPCAM -30 Actin -40