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Imaging of laser-excited
skin autofluorescence
bleaching rates.
Kristine Rozniece, Ilona Hartmane
Clinical Center of Skin and ST Diseases, Riga, Latvia
Janis Lesinsh, Janis Spigulis, ASI, LU, Riga, Latvia
European Social Fund
"Biophotonic research group"
Nr.2009/0211/1DP/1.1.1.2.0/09/APIA/VIAA/077
BADV,Riga, 08.09.2011.
Autofluorescence of Skin

An optical diagnostic technique in vivo that
differentiates skin lesions from healthy tissue,
based on measurements of the fluorescence
intensity emitted by native fluorophores present
in different concentration in skin tissues.
 This autofluorescence is due to the absorption of
the exciting radiation by fluorophores
(tryptophan, collagen, elastin, NADH, flavin,
lipofuscin, melanin, hemoglobin) that results in
emission of radiation at higher wavelenghts.
Autofluorescence Photobleaching
 The
process of decreasing the
fluoroscence intensity during long-term
optical excitation.
 Is caused by degradation of the skin
endogenous fluorophore molecules.
 The florophores that emit under bluegreen excitation are NAD, kreatin, as well
as the dermal collagen and elastin.
Aim of Research
 In
this study there are presented some
comparative results of using green light
(532 nm), low power cw laser as excitation
source for cutaneous autofluorescence
investigations and evaluation of
autofluorescence properties of normal skin
and different skin pathalogies in vivo.
Materials and Methods
 The
round spot of 4 mm in diameter was
selected on skin of the inner forearm of
healthy volunteer, and the spot was
irradiated by 532 nm cw low power laser
for 1 min. (n= 30)
 Patients with benign different melanocytic,
vascular, hyperkeratotic and fibrotic
lesions of skin (n=74)
 Patients with BCC (n=2)
Junctional NMN
Compound NMN
Angioma
Seborrheic keratosis
Dermatofibroma
BCC
gersed3 - AF intensistāte pie 600nm (532nm lāzera ierosme)
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AF Intensity (normalized by max)
AF in different skin pathologies
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lentigo
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angioma
blue nevus%
junctional NMN
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Time (Seconds)
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Distribution of patients
BCC
dermatofibroma
seborrheic keratosis
angioma
lentigo
congenital nevus
dysplastic nevus
junctional NMN
blue NMN
compound NMN
Statistical Analysis
the Shapiro –Wilk test min,
max, and average intensity of AF was
not normally distributed in all patient
groups.
 Due this reason we used the Mann –
Withney test for further analysis of
differences.
 According
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Conclusions

Taking in consideration that various shapes of
autofluorescence in cases of different skin
pathologies were clearly different, we have
found statistically significant differences in min,
max and average only between groups with
junctional and compound NMN.
 BCC shows the highest AF intensity in
comperasion with other pathologies, but more
patients should be involved to get statistically
significant results in this study.
Thank you for your
attention!