Transcript FLIM

Fluorescence Life-Time Imaging
Microscopy
ESF projekts Biofotonikas pētījumu grupa
Nr.2009/0211/1DP/1.1.1.2.0/09/APIA/VIAA/077
Inesa Ferulova
DAUGAVPILS UNIVERSITĀTE
Dabaszinātņu un
matemātikas fakultāte
G. Liberta Inovatīvās
mikroskopijas centrs
Wavelength: 690 nm – 1040 nm
Power: 2.6 W
Repetition rate: 80 MHz
Wavwlength, Power:
• 633 nm, 15 mW, 4 mW
• 458 nm, 200mW, 30mW
• 476 nm, 200mW, 30mW
• 488 nm, 200mW, 30mW
• 496 nm, 200mW, 30mW
• 514 nm, 200mW, 30mW
FLIM
x,y plaknē
x,y plaknē
Fluorescence
x,z plaknē
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Piko sekunžu lāzers
Fotonu skaitītājs
TCSPC (Time-correlated single
photon counting)/Pārveidotājs
Sinhronizators / no lāzera uz pārveidotāju dod
signālu ka sakas jauns mērījums
Time-correlated single photon counting
Laser
controller
interface
Pico-second
laser
Optical fiber
Simple Tau 130 Lifetime analyzer
system
Sample
PC equipped with software
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Pico-second Laser PiLas
PiLas control EIG1000AF - repetition rate: 80 MHz
Advanced Laser Diode Systems A.L.S. GmbH
Simple-Tau 130:
Ultra-fast time-correlated single photon counting system based on SPC-130, consisting of:
- Notebook Computer with software
- 4 slot PCI-Box with connection cable and Interface-card for notebook computer
- DCC-100 Detector Controller
- SPC-130 TCSPC Module
Photon counter HPM-100-40-CMOUNT:
Hybrid PMT Detector Head for Photon Counting, 300-720nm.
Becker & Hickl GmbH