Transcript Rotifers – their role and production requirements
Rotifers
their role and production requirements
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Mass production Mobility Tolerance Bio-encapsulation
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Reproduction Size
Life cycle
B. plicatilis
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Rotifer production – optimal culture and enrichment conditions
Tank: Temp.: Salinity: Oxygen: Any Size/Shape 25-27 °C 18-25 ppt 5-7 ppm
(2-6 m (Pure Oxygen) 3 ) (Preheated Water/Heat Exchange)
pH: 7.5-8.5
NH 3 /NH 4 +: < 10 mg/l NH 3 : < 1 mg/l Water: Filtered (1
m), UV, Chlorinated/Neutralized Aeration - Reasonably Strong - Airstones Illumination: Low Light Conditions Floc traps Batch Culture: 3-5 days
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Rotifer production in 3m3 tanks Live food aquaculture training course www.aquatrain.org
Low density rotifer culture
Sea bream General assumptions
1) Av. daily rotifer consumption/m3 larval volume (M) 2) Rotifer production volume/100M daily production (m3) 3) Period of rotifer feeding (days) 4) Culture Selco needed per 100M rotifers produced (kg) 5) Culture Selco price (drs/kg) Requirements No of larval batches Initial larval rearing vol (m3) Daily rotifer requirement (M) Rotifer production volume (m3) No of 2.8m3 tanks needed No of 4m3 tanks required Total rotifer production volume (m3) Total Culture Selco requirement (kg) Cost of Culture Selco (Mdrs/yr) Annual production target (2g juveniles) 2.000.000 4.000.000 5 62 615 9 3 0 8 123 1,38 5 123 1.231
18 7 0 20 246 2,77 10 1,5 20 0,2 11234 6.000.000 5 185 1.846
28 10 0 28 369 4,15 10.000.000 5 308 3.077
46 16 0 45 615 6,91 Live food aquaculture training course www.aquatrain.org
Rotifer culture using algae
excellent results with algae but expensive
time consuming (culture of algae + counting)
availability of algae
quality of the rotifers dependent on quality of the algae
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Rotifer culture using algae and yeast
variable results in rotifer survival depending on the quality of algae and freshness of the yeast
unexpected mortalities (total crashes) due to lack of standardisation
variability in rotifer quality (nutrition, microbiology)
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Batch culture of rotifers on algae and yeast
Culture period
6-10 days
harvest drain culture water Re-inoculation
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f e e d i n g
Medium density rotifer culture
Sea bream General assumptions
1) Av. daily rotifer consumption/m3 larval volume (M) 2) Rotifer production volume/100M daily production (m3) 3) Period of rotifer feeding (days) 4) Culture Selco 3000 needed per 100M rotifers produced (kg) 5) Culture Selco price (drs/kg) Requirements No of larval batches Initial larval rearing vol (m3) Daily rotifer requirement (M) Rotifer production volume (m3) No of 2.8m3 tanks needed No of 4m3 tanks required Total rotifer production volume (m3) Total Culture Selco 3000 requirement (kg) Cost of Culture Selco (Mdrs/yr) Annual production target (2g juveniles) 2.000.000 4.000.000 5 62 615 2 3 0 8 123 0,00 5 123 1.231
5 7 0 20 246 0,00 6.000.000 5 185 1.846
7 10 0 28 369 0,00 10.000.000 5 308 3.077
12 16 0 45 615 0,00 Live food aquaculture training course www.aquatrain.org
10 0,4 20 0,20
Rotifer growth comparison CS v’s CS3000 CS 3000-MD vs. CS-LD
CS 3000-MD CS-LD 1800 1600 1400 1200 1000 800 600 400 200 0 0 1 2
Time (days)
3 Live food aquaculture training course www.aquatrain.org
Specific Growth Rate in MD batch culture
SGR in MD batch culture: CS vs. CS 3000
0.5
0.4
0.3
0.2
0.1
0 CS 3000 CS Live food aquaculture training course www.aquatrain.org
Method of determining rotifer growth and condition – mean specific growth rates SGR = ln (Final Number)-ln (Initial number) No. of days of culture ln = natural log Useful for: Following feeding experiments Quantitatively assessing culture stability Live food aquaculture training course www.aquatrain.org
Rotifer production methodologies Culture Selco 3000 and O2 Live food aquaculture training course www.aquatrain.org
Rotifer Production
M M2 30% A M
: Masters
A
: Culture (2-6 m
3
)
B
: Inoculum (500 R/ml)
70% C
: Enrichment (500-1000 R/ml) Larval Tanks
C B
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Culture Selco 3000 strategy
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Traditional rotifer batch culture
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High density rotifer production system
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Rotifer harvesting Live food aquaculture training course www.aquatrain.org
Harvesting of rotifers Live food aquaculture training course www.aquatrain.org
Transfer of rotifers Live food aquaculture training course www.aquatrain.org
Recirculated ultra-high density rotifer culture ~5000 rotifers + /ml Live food aquaculture training course www.aquatrain.org
Commercially available rotifer and artemia cultivation and enrichment products Live food aquaculture training course www.aquatrain.org
Fatty acid enrichment levels in Rotifers
Enriched rotifers
Nannochloropsis Nannochloropsis sp.
Chlorella Isochrysis sp.
Protein Selco Super Selco DHA Super Selco
mg/g
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