Transcript Rotifer culture systems

Rotifer culture systems
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Rotifer Batch Culture
Low density in large tanks 3 to 10 m3
inoculate at 50/ml increasing to 250/ml
Medium density in tanks 2 to 5 m3
inoculate at 200/ml increasing to 800/ml
High density in tanks 2 to 3 m3
inoculate at 500/ml increasing to 2000/ml
Ultra high density in 1 to 3 m3
inoculate at 1000/ml increasing to 3500/ml to
10,000/ml
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Batch culture of Rotifers
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Batch culture of Rotifers
500/ml
800/ml
1200/ml
• 4 day cycle
• 2000/ml - 500/ml = 1500/ml = 1.5 bil/m3
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2000/ml
Semi-continuous culture of Rotifers
Feed, B12
sterlised water
• 10 to 15 % harvested daily then taken for enrichment
• each tank operated for up to 10 days
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Semi-continuous culture of Rotifers
2000/ml
2000/ml
2000/ml
2000/ml
• 10 to 15 % harvested daily (12.5%)
• 250/ml harvested daily x 4 tanks = 1000/ml= 1 bill/m3
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Continuous culture of Rotifers
Recirc
2000/ml
2000/ml
2000/ml
2000/ml
Biofilter
Prot Skim
UV
• 20 to 25 % harvested daily (22.5%)
• 450/ml harvested daily x 4 tanks = 1800/ml = 1.8 bill/m3
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High density continuous production
Recirculation
system
Pump
Biofilter
Protein skimmer + O3
UV steriliser
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High density rotifer production
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Collection system
Rinsing
water
Rotifers
Aeration
ring
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Improved filter system
Rinsing
water
Rotifers
Aeration
ring
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Continuous rotifer production system (CROPS) Design
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Culture tank
Settling tank (pump)
Skimmer loop (ozone, Pskimmer, U.V.)
Biofilter
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Design schematic
Bypass biofilter
Filter 30 um
Airlift
Bioflow
UV
Skimmer
Filter 60 um
P
Ozone
Bypass skimmer
Low Pressure Air
Oxygen
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Construction
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Ozonator and protein skimmer
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Continuous rotifer production system (CROPS) Design
 Culture tank
 Settling tank (pump)
 Skimmer loop (ozone, Protein
skimmer, U.V.)
 Biofilter
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Improved CROPS Design
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Paste feeding system
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