Transcript 9 BG UNM TVDC 2007 Meeting Tues session 100907

TVDC Milestone Completion
Reports
Barbara Griffith, M.S
TVDC Project Director
10/9/2007
MS Completion Reports:
Purpose
• “Stand Alone” Report for the Milestone
• Summarize the Milestone’s objectives,
methods, original data, interpretations and
conclusions
• Equivalent to a mini-manuscript and could be
developed into an article for publication
• Milestone Completion Reports are required by
the TVD Contract
• Monthly Technical Report Format: not working
MS Completion Report
Template: Content
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Signature Page
Abstract/Executive Summary
Milestone Objectives
Methods, Critical Reagents and SOPs
Salient Original Data, Results, Interpretation,
Quality Control
• Deliverables Completed
• Appendices
Abstract/Executive Summary
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•
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Summary= abstract in journal publication
Brief statement of goals
Succinct statement of results
Overall interpretation of data, without
reviewing the data itself
• Concise conclusions for the milestone
Milestone Objectives
• These are the objectives listed in the
Statement of Work and Monthly Technical
Reports for the milestone
• UNM will pre-fill this text into the Milestone
Completion Report templates
Methods, Critical Reagents and
SOPs
• Methods: summarize methods and include
details necessary to re-perform critical
experiments
• Critical Reagents: list reagents, sources
and critical lot numbers
• Standard Operating Procedures: protocols
followed in your laboratory written in
quality format ( format presented by Marlene Hammer at the
UNM/DVC/NIAID meeting in Woods Hole 2006; “LVS growth in
Chamberlain’s” is an example)
Salient Original Data and Results
• Include only positive and negative data that
contribute to the completion and understanding
of the Milestone. Will be a judgment call.
• Do NOT include all original data from all
experiments performed
• Example: optimizing ASU IVT product synthesis
– Performed pilot studies to test many variables and
to optimize the synthesis : His tags locations, feeds,
template spikes, purification methods, etc.
– Summarize the variables tested in pilot studies,
describe which are significant, and only present
original data on the critical variables that led to
optimal IVT product quality and quantity.
Salient Original Data and Results
• Organize data by the goals or deliverables for
the milestone
• Insert original data into the text to enhance the
flow of the report. Insert data as an appendix if data interrupts
the flow of the report (e.g. multi sheet excel workbooks)
• Provide a figure/table legend to briefly describe
how the experiment was performed (similar to a figure
in a publication)
• Annotate tables and figures with notebook
locations and electronic locations. Provide a crossreference if the final locations differ from locations cited in the Monthly
Technical Reports
• Include QC that supports conclusions (e.g. DNA
sequence verification, experiment reproducibility, protein expression
verification by Western blot)
Data Interpretation and
Quality Control
• Interpretation
– Provide interpretation of data and results
– Address unresolved questions, issues or problems relevant to
the milestone
– Articulate possible next steps to advance the understanding of
the data and results
• Quality Control
– Include QC data that supports the repeatability of
the data (e.g. number of times that experiments were repeated,
statistical analysis of variance, replicates
– Include QC data and information that supports the
interpretation and significance of the results
• (e.g. DNA sequence verification, experiment reproducibility,
protein expression verification by Western blot)
Deliverables Completed
• Review deliverables stated in the SOW from the
subcontract. (e.g. reagents, SOPs, MS Completion Reports)
• Reagents Generated
– Types: bacterial strains, plasmid constructs,
polypeptides, RNA/DNA, tissues
– type, name, # of vials, concentration, storage media,
storage location at subcontractor’s institution, protein
or DNA sequence, etc
• Reagents Shared with UNM and other
Subcontractors:
– Date, quantity, institution, location at institution
Appendices
1. List of Original Data Tables & Figuresquick reference guide to original data locations
2. Milestone Completion Report
Assessment Criteria (from Strategic Work Plan)tracks review of reports
3. Original Data- if too disruptive to flow of the MS
Completion Report
4. SOPs- procedures written in quality format
Next Steps
• UNM will provide the MS Completion
Report Templates to subcontractors
• For previously submitted MS Completion
Reports, UNM will “pre-fill” as much as
possible
– ASU- MS 25, 32
– UTSA- MS 16, 39, 48
• Provide feedback to UNM
– Suggest improvements
Questions after Presentation
• Alex B: Can data be saved cumulatively in an
excel sheet or does the data need to be
captured in time as snapshots with each monthly
report?
• Barbara/Vicki/Marlene: Data can be saved
cumulatively in an excel workbook
• Vicki: The MS Completion Reports should stand
alone and make the monthly technical reports
virtually obsolete, though the month technical
reports will be saved for reference.