Transcript lecture_3_456_2015.pptx

Biol 456/656
Molecular Epigenetics
Lecture #3
Aug 28, 2015
Videos explaining Illumina Hi-Seq
https://www.youtube.com/watch?v=d2AxXv_6UTQ
https://www.youtube.com/watch?v=HMyCqWhwB8E
See Brown_Celniker_Nature_2014.pdf
http://www.nature.com/nature/journal/v512/n7515/full/nature12962.html
Figure 1
Figure 1
What is CAGE?
Figure 1
Dystrophin 3’ end
Figure 1
Go to: chrX:4,892,228-4,981,117
Figure 2
Figure 2
chrX:15,753,078-15,761,360
Per cent spliced in index
Figure 2
Figure 3
Figure 3
Figure 4
Figure 5
Figure 6
Homework: Read “Diversity and dynamics of the Drosophila transcriptome”
1. Draw or explain in words “gene models” illustrating:
A. First exon variant alternative splicing
B. Convergent transcription
C. Antisense transcription
2. Use the UCSC genome browser to find examples of the above events (1A, 1B, 1C)
and take screenshots of these. Use arrows and text boxes to point out relevant information.
3. Identify 3 aspects of the paper (a figure, technique, approach or statement made)
that you completely do not understand.
4. Explain, briefly, how you attempted to read the paper.
5. What would you score this paper out of 10?
Email me your answers prior to Monday’s class in a word, pages or powerpoint file.