Transcript Pruitt Austin sol 2014 sm

Butanol
Producing
E. Coli and
Algae biofuels
Austin Pruitt
The Plan
Chlorella Vulgaris
Bump Array
Sorts Particles by size
Aids in evolutionary acceleration
E. Coli
Death Galaxy
The Death Galaxy is a
microchip device with
interconnected wells
and gradient
Mimics heterogeneous
microenvironments
Can result in rapid
evolution
Evolutionary Dynamic
Principles

Stress gradient and microenvironments should
yield rapid evolution

Low stress allows reproduction

Mutated cells climb the gradient

Small population size in wells allows faster
fixation
E. Coli Resistance to Cipro
Progress so far
Next Steps
 Begin
UV and Bump array testing
 Run several death galaxies simultaneously
 Attempt to evolve bacteria/algae to
thrive in different conditions
Conclusion
 Gained
many skills through this
experience
 Ongoing research
 Better understanding of evolutionary
dynamics, and fluid mechanics
Thank you!
Sources
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http://www.princeton.edu/physics/puchalla/research/microfluidics
/BumpArray_1.jpg
http://harmonicarts.ca/wpcontent/uploads/2013/04/chlorella_vulgaris_beij_1_0.jpg
http://krisdedecker.typepad.com/photos/uncategorized/2008/04/
04/algae1.jpg
http://ak2.picdn.net/shutterstock/videos/4077487/preview/stockfootage-moscow-june-cars-stands-in-traffic-jam-on-the-city-centerjune-moscow-russia.jpg
http://innovativesolarsystemsllc.com/wp-content/uploads/solarpower-generator.jpg
http://s.hswstatic.com/gif/hydropower-plant-usbr-hoover-1.jpg
http://cleantechnica.com/files/2014/04/wind-turbines17.jpg
http://www.prism.princeton.edu/PRISM_cleanroom/images/prismlo
gosmall.jpg