Transcript cheung_tiffany_sol _sm

Field Studies of
Phytoplankton &
Laboratory Studies of
Marine Diatoms
(Sigman, Morel Lab Group)
Marine Diatoms
• What are marine diatoms and its research significance?
(Hanna Mossfeldt, nordicmicroalgae.org)
• Model organism for lab studies, Thalassiosira weissflogii (TW)
• Effects of TW in response to light intensities
• Effects of TW’s protein production over 12:12 diel cycling
• Effects of TW in response to increasing temperature
Particular Proteins of Interest
Photosynthesis: CO2 + H2O  (light E) “CH2O” + O2
Chlorophyll a
RuBisCo
Eichhorn, et al – Plant Biology
Jones, M.
1.6
8
1.4
7
1.2
6
1
5
0.8
4
0.6
3
0.4
2
growth rate (day^-1)
rubisco (%TP)
0.2
1
0
0
16.5
18.5
20.5
22.5
24.5
Temperature (C)
26.5
28.5
30.5
Rubisco Concentration (% of total protein)
Growth Rate (day^-1)
Temperature vs. Growth Rate vs. Rubisco
Further Research
 Junior Proposal & Research Paper
1) Refine Temperature vs. Growth Rate vs. Rubisco Concentration growth curves
1) Define temperature range to examine – low to optimum
• growth rate is increasing
• rubisco concentration is decreasing
3) Quantifications + Activity Measurements
Quantifications
• growth rate
• chlorophyll a
(parameter)
• protein, Rubisco, TWCA
Activity Measurements
• NPP (whole cells, C-14)
• Rubisco activity (C-14)
• O2 evolving activity
Other Measurements
• Kc = (1/2Vmax), rbc
• Ki
• Ω