Transcript Heat shock proteins 90
The Department of Biochemistry of Medical Faculty Presents now
MOLECULAR CHAPERONES
Edited by Attila Sandor
one dimensional information L. Fig.3-16, p.89
three dimensional information
The model proteins for classic study of developing the three dimensional structure, that for folding. (Anfinsen) Str., Fig. 7.1, p.172
Str. Fig. 3.53, p. 65
Str. Fig. 3.54, p. 66 Scrambled
The three dimensional structure is dictated entirely by the amino acid sequence
Str., Fig1.6 p.7
L.Fig.4-29,p. 149
Str. Fig. 3.57, p. 6+8
Role of chaperones in the thermodynamic point of view (a) The folding of the unfolded protein (U) to the correct (C) form is favored thermodynamically.
Chaperone is not necessary.
(b) The folding of the unfolded protein (U) to the incorrect (I) form is favored thermodynamically.
Chaperone is necessary.
Ellis R.J., Annu. Rev. Biochem., 1991
The idea of
molecular
chaperones
Named after the human chaperone: “usually an elderly woman who accompanies a young unmarried lady
to prevent not proper interactions
with other people.
Molecular chaperones: proteins assisting folding of nascent polypeptides, by
preventing wrong folding
.
Molecular chaperones catalyze the formation of correctly folded, functionally active, native proteins, but they are
not part of the product.
Expression of many chaperon is induced by stress, such as by heat, because during heat-stress the probability of wrong folding is higher, therefore cells need more protection. These chaperons are called
heat shock proteins (HSP’s).
Author`s slide
Representative members of the Chaperone family Nucleoplasmins
Nucloplasmin NucleoplasminS
Chaperonins
chaperonin 60, groEL chaperonin 60, groEL chaperonin 10, groES (mitochondrial, bacterial) may use ATP
Heat shock proteins 70 (
Hsp70)
Heat shock proteins 90
(Hsp90)
Ellis R.J., Annu. Rev. Biochem., 1991
Role of nucleoplasmines: assably of chromatin
DNA + Histone DNA+ Nucleoplasmin + Histone aggregate Nucleosoma (DNA + Histone) Nucleoplasmin Author`s picture
Representative members of the Chaperone family Nucleoplasmins
Nucloplasmin NucleoplasminS
Chaperonins
chaperonin 60, groEL chaperonin 60, groEL chaperonin 10, groES (mitochondrial, bacterial) may use ATP
Heat shock proteins 70 (
Hsp70)
Heat shock proteins 90
(Hsp90)
Ellis R.J., Annu. Rev. Biochem., 1991
RUBISCO and the chaperonins
When removing guanine Rubisco denaturated with guanine at +25 o C very poor recovery Rubisco denaturated with guanine at +10 o C good recovery Rubisco denaturated with guanine at +25 o and chaperonin 60, chaperonin 10, ATP, Mg ++ were added C 80% recovery Rubisco: ribulose 1,5-bisphosphate carboxylase-oxygenase Author`s slide
L.Fig.41-31, p.152
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Attila Sandor The honored audience has the opportunity now to
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