Transcript microscopes

Microscopes!!!
How will you view cells in this
class?
• Using microscopes
• Proper way to
carry microscope
– Carry with cord
wrapped around
– Hold by arm and
base
Parts of a Microscope
Objectives
• Start with scanning (lowest)
objective (4X)
• Move on to lower and higher
powered objectives
Eyepiece Ocular
Lens
Total
Magnification
Scanning
(always start
here!)
4X
10X
40X
Low
10X
10X
100X
High
40X
10X
400X
Learning to Use the
Microscope
To view an object:
– Start with the stage at the LOWEST height (coarse adj
knob) and the SCANNING objective in place
– Clip your slides in with the stage clips.
– Turn on the microscope.
– Adjust the coarse adjustment knob until you can see the
sample.
– Adjust the fine adjustment know until the sample can be
seen clearly.
– REMOVE the slide by turning the coarse adj knob to
lowest position.
– When finished, wrap cord on itself! Not on microscope.
Making a Wet Mount
1. Wet specimen if necessary. Place on center or well
of slide.
2. Cover with coverslip.
3. Drop stain on one side of coverslip.
4. Place piece of paper towel on OTHER SIDE of
coverslip.
5. Wick water through, remove slide. Place on
microscope.
Making Scientific Drawings
1.
Obtain a Petri dish to make
“field of view”.
2. Drawings can be in
pencil/colored pencils.
3. Must include clear, proper
labels that start outside the
circle.
4. Must include magnification.
5. Animal cells should always
have- TITLE and…
Cell membrane, nucleus, nuclear
membrane, cytoplasm, organelles
Plant cells: all above and
chloroplasts, cell wall
6. Don’t just LOOK! You want to
OBSERVE!
Quick Review
1. Show how to properly hold a microscope.
2. What must you always move DOWN to
begin?
3. What OBJECTIVE must you start with to
begin?
4. What is the magnification of the ocular
lens (eyepiece)?
5. If you are viewing a specimen magnified a
total of 400 X, what objective are you
using?