Transcript Microscope

TOPIC: Microscope
AIM: What are the
parts of a microscope?
Microscope • Instrument that produces a
larger image of an object
(specimen) using lenses
Lens • Piece of curved glass
• Causes light rays to come
together or spread apart
• Magnify an image
Simple
Microscope
• Uses 1 lens and light
to magnify the
specimen
Compound
Light
Microscope
• Uses 2 lenses & light
to magnify specimen
• Need stain (dye) to
see it
Blood
Cheek Cells 40x
Onion Cells 10x
PARTS OF A MICROSCOPE
1.
• Ocular lens (usually 10x)
eyepiece • Lens you look through
2.
• Contains lenses
Objective• Low, medium, high power
lenses
Low Power
High Power
• Low power objective = used to
locate the specimen on the slide
• Larger field of view (See more
of the entire object)
• High power objective =
• More magnification
• Smaller field of view(see less of
the entire object)
3.
• Holds & rotates obj.
lenses
Nosepiece
4.
• Connects eyepiece to
Body Tube nosepiece
5. Arm • Supports body tube
6. Base • Bottom = support
7. Stage• Where slide is placed
8.
Stage
Clips
• Hold slide in place
• Illuminates specimen,
9.
provides light
Mirror/
light
10.
• Controls the AMOUNT
Diaphragm of light used
• Larger focusing knob
11.
Coarse
Adjustment
Knob
• For focusing under LOW
POWER ONLY
• If used under high power,
objective lens and/or slide
can break
• Sharpens image
12.
• Focusing for High Power
Fine
Adjustment
Knob
Most
• Electron microscope:
magnifies up to 1 million
powerful
times
microscope
• Uses beam of electrons
instead of light
Fly
Spider
Termite head
Tapeworm
Blood
• Scanning Electron
SEM Microscope
• Produces real, 3D image
• Only scans surface
• magnifies 20x - to 200,000x
TEM
• Transmission Electron
Microscope
• Produces 2D image of thinly
sliced organism
• magnifies 1,000x 1,000,000x
Phase
• View living specimens
without staining
Contrast
Microscope
Light microscope
Phase contrast
microscope