Transcript Document 7744917

VISCOSITY
Transport property defined as the
retarding effect of slower layers of
a fluid on an adjacent faster layer.
VISCOSITY
• Viscosity is a transport property that is
observed when there is a momentum or
velocity gradient.
• It is defined as the retarding effect of a
slower layer of a fluid on an adjacent
faster layer.
• Flux = Jx α -dv/dx where the
proportionality constant = η = viscosity
coefficient or constant ( g/(cm-s)
Experiment
1. Measure viscosity by measuring time (t) it
takes a fluid to fall between two marks of
viscometer. ηρ = Bt where ρ = fluid
density and B = viscometer constant.
2. Determine B using water
3. Determine η of BSA as a function of its
concentration
Experiment (2)
4. Determine effective spherical radius of
BSA protein.
5. Repeat steps 3 and 4 for denatured BSA.
Two-Day Expt
• Day 1: Native BSA: make stock solution
and its dilutions; measure η = f(c).
Denatured BSA: make stock solution
• Day 2: d-BSA: make dilutions and
measure η = f(c).