Transcript INFLUENZA TRANSPORT MEDIUM MEDIA PREPARATION TEAM Miguel Garza, Supervisor Martha Thompson, Viral Isolation Laboratory.

INFLUENZA
TRANSPORT MEDIUM
MEDIA PREPARATION TEAM
Miguel Garza, Supervisor
Martha Thompson, Viral Isolation Laboratory
Ingredients
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Tryptose Phosphate Broth
Gelatin
Penicillin 250,000 units per mL
Streptomycin Sulfate 0.25 grams per
mL
Distilled Water
Preparation
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Combine in a 4 liter flask
Dissolve by stirring
Autoclave 30 minutes at 121° C
Add aseptically Penicillin 250,000
units and Streptomycin Sulfate 0.25
grams
Dispense aseptically 3.0 mls to
1998(aprox.) sterile tubes
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Label each tube: INFLUENZA TRANSPORT,
93515, lot number, expiration and Texas
Dept. of State Health Services, Austin, TX
78756
Expiration date is 12 months from date of
preparation
Store in Media Production Freezer at -25
°C
Deliver 7 tubes to Virology Team for
quality control
QC of Transport Media
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Sterility
Toxicity
Growth assay
 Storage at -70° C for 72 hrs
 Storage at 4° C for 24 hrs
 Undilute, 1:5, 1:10
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Parallel testing old/new lots
Viral Transport Media
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Viral transport medium
Hanks balanced salt solution
Tryptose-phosphate broth
Sucrose-phosphate broth
Cell culture medium
Veal infusion broth
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Supplement with a protein or stabilizer, bovine
serum albumin or gelatin at 0.5 to 1%.
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Antibiotics