Transcript INFLUENZA TRANSPORT MEDIUM MEDIA PREPARATION TEAM Miguel Garza, Supervisor Ingredients      Tryptose Phosphate Broth, 177g Gelatin, 30g Penicillin 250,000 units per mL, 6mls Streptomycin Sulfate 0.25 grams per mL,

INFLUENZA
TRANSPORT MEDIUM
MEDIA PREPARATION TEAM
Miguel Garza, Supervisor
Ingredients
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Tryptose Phosphate Broth, 177g
Gelatin, 30g
Penicillin 250,000 units per mL, 6mls
Streptomycin Sulfate 0.25 grams per
mL, 6mls
Distilled Water, 6000mls
Preparation
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Combine in a 4 liter flask
Dissolve by stirring
Autoclave 30 minutes at 121° C
Add aseptically Penicillin 250,000
units and Streptomycin Sulfate 0.25
grams
Dispense aseptically 3.0 mls to
1998(aprox.) sterile tubes
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Label each tube: INFLUENZA TRANSPORT,
93515, lot number, expiration and Texas
Dept. of State Health Services, Austin, TX
78756
Expiration date is September 30, 2010
Store in Media Production Freezer at -25
°C
Deliver 7 tubes to Virology Team for
quality control
Viral Transport Media
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IT-53 LOT 1, 1736
IT-53 LOT 2, 1799
IT-54, 1871
IT-55, 1778
IT-56, 1728
IT-57, 1772
IT-58, 1812
IT-59, 1742
IT-60, 1733
Viral Transport Media
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Total 15,971 units made
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Lots available: IT-58, IT 59 and IT-60
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Number of units available, 4868
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Making 4 new lots before October 1, 2010
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7000 more units
QC of Transport Media
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Sterility
Toxicity
Growth assay
 Storage at -70° C for 72 hrs
 Storage at 4° C for 24 hrs
 Undilute, 1:5, 1:10
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Parallel testing old/new lots
Viral Transport Media
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Viral transport medium
Hanks balanced salt solution
Tryptose-phosphate broth
Sucrose-phosphate broth
Cell culture medium
Veal infusion broth
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Supplement with a protein or stabilizer, bovine
serum albumin or gelatin at 0.5 to 1%.
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Antibiotics