Transcript PERKIN-ELMER MODEL 200 UV-VIS SPECTROPHOTOMETER OPERATING INSTRUCTIONS INSTRUMENT WARM UP • Turn the power switch on.

PERKIN-ELMER MODEL 200 UV-VIS
SPECTROPHOTOMETER
OPERATING INSTRUCTIONS
INSTRUMENT WARM UP
• Turn the power switch on. Turn on the tungsten lamp
(W lamp). The tungsten lamp is used up to 370 nm.
Allow the lamp to warm up for a few minutes. (30
minutes are necessary for stable operation).
• Set the source selector to the Visible lamp (out).
• Set the slit to the minimum, 0.2 nm. This setting gives
sufficient resolution for recording spectra of gas-phase
samples. Set the response to MEDIUM for now.
%T and Absorbance Calibration
• Depress the %T button. Insert the 0%T
shutter (large plate) along the side of the
sample compartment, blocking the sample
beam.
%T and Absorbance Calibration
• Set +00.0 on the digital display using the 0%T
control. Turn control enough to see –00.0
then turn back until +00.0 just appears.
• Remove the 0%T shutter. Set +100.0 with the
100%T/ ABS. Control.
Absorbance Spectrum
• Push the 0-2 Abs button to switch to
Absorbance readout.
• Set spectrophotometer SCAN SPEED selector
to 30 nm/min. The drive gears should be
arranged as shown below.
Absorbance Spectrum
• Set the RESPONSE switch to FAST, to follow the
sharp gas-phase peaks.
• Place the sample cell in the sample
compartment.
Absorbance Spectrum
• Manually set the initial, longest wavelength, in
the monochromator.
• When setting wavelengths, always turn the
WAVELENGTH control from longer to shorter
wavelengths to avoid backlash in the gears for
best reproducibility.
• Click the Start Green button on LoggerPro
• Switch the Scan Box switch to ON
• After data collection click the red Stop button
in Logger Pro and turn off the Scan Box switch