Transcript Ⅰ KRIBB* Introduction Ⅱ Proposed Agendas Ⅲ Contact Information *KRIBB: Korea Research Institute of Bioscience and Biotechnology.

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KRIBB* Introduction
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Proposed Agendas
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Contact Information
*KRIBB: Korea Research Institute of Bioscience and
Biotechnology
KRIBB Introduction
History
1985. Feb. : Founded as a Genetic Engineering Center
1995. Mar. : Changed name to KRIBB
2001. Jan. : Became an independent legal entity
Personal
1,170(as of Apr 2012)
- 331 full-time, 553 temporary (Post Mas., Post Doc)
- 286 students(M.S. & Ph.D.)
Budget
Budget $120M
- 47% from government directly
Daejeon Headquarters : Basic Biological Research
Campus
- Genomics, Proteomics, Biological Resources, Bionanotechnology
Ochang Campus : Drug Discovery, Biotherapeutics
- Natural Products, Immunotherapy
Jeonbuk Campus : Innovative Biomaterials
- Applied Microbiology, Bioindustrial Process Development
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Cooperation Overview
Brazil
1. Development of Cosmetics & Life
Cares from Biodiversity by Dr. HoYong Park
Biodiversity
Human
Resources
Technology
Korea
(KRIBB)
2. Development of Bio-ethanol
a) Development of Core Technology
for 2nd Bio-ethanol by Dr. KwangHee Son
b) Using the Sugarcane Bagasse by
Dr. Chul Ho Kim
Knowledge Transfer
R&D Development and Cooperation
Biz Development Practices
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1. Development of Cosmetics & Life Cares from Biodiversity
Goal
• Development of cosmetics and life cares using the
ingredients from biodiversity
Contents
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Screening/selection of bioactive substances
Analysis/determination of natural ingredients
Registration/formulation of active ingredients
Production of commercial products
To sell on the global market & share profit
[Brazil] … Biodiversity, traditional knowledge etc.
[Korea] … Commercialization technology, market
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1. Development of Cosmetics & Life Cares from Biodiversity
Actions Plans & Procedures
1. Finding common interest at co-steering committee
between Korea and Brazil
2. Matching the Brazilian counterpart
Korean principal counterpart : KRIBB (and associated companies)
3. Confirmation of the cooperation R&BD project
Discussing the details between co-workers, a MOU signing etc.
4. Performance of co-R&BD project by two countries
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Screening/selection of bioactive substances
Analysis/determination of natural ingredients
Registration & formulation of active ingredients(CTFA,FDA etc)
5. Production & Sale of the products for the global market
* KRIBB has a strong commercialization technology and
competitiveness for cosmetic ingredients from biodiversity.
‘Arazyme’
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Achievements
of my team
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Experience 1 : Mutual visiting and Seminar
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Experience 2 : Collaboration between Korea & Brazil
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2. Development of Core Technology for 2nd Generation Bio-ethanol
Agenda
 Non-starch carbohydrate biomass as a substrate for bio-ethanol
- solution for bio-recalcitrance of biomass
AgFlation
 Enzyme (hemicellulase) to break down the core structure of wood part
 Cooperation; Enzyme tech. (Korea) and biodiversity (Brazil)
Contents
 Cloning skill (based on DNA walking) of Korea and abundant gene pool
of Brazil biodiversity can make a new cooperation model in enzyme field.
- (Brazil) Canopy bioresources containing genetic diversity those are accessible to
recalcitrance substrate.
- (Brazil) Primary screening of enzyme group and library construction
- (Korea) Symbiotic microbes from screened bioresources
- (Brazil-Korea) Molecular biology tool and resource for functional study
Effects
 Positive cooperation model for the international ABS issue
- Green technology and green economics
 Share global enzyme markets and technologies.
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2. Development of Core Technology for 2nd Generation Bio-ethanol
Hemicellulase for 2nd generation bio-ethanol:
Longicorn derived symbiotic microorganisms for the
expression/production of new enzymes
Biodiversity
Resource that make sense
Symbiotic bacteria
Gene/Protein
Expression system / Scale-up
Enzyme production
•(Wood, corn
stover에 적용하여
바이오에탄올 생산)
•(feed enzymes)
Application
Cloning,
Expression,
Purification
(Process)
Degradation/Biorefinery of
cellulosic/hemicellulosic
materials
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2. Development of Core Technology for 2nd Generation Bio-ethanol
Hemicellulase for 2nd generation bio-ethanol :
Industrial enzymes
(source: The Novoymes report)
 Technical Enzyme Development For Bio-Ethanol
Bio-Ethanol using starch
(1st Generation materials)
Bio-Ethanol using hemicellulogic feed stock
(2nd Generation materials)
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3. Development of Bio-ethanol using the Sugarcane Bagasse
Background
Biomass Based Economy
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Transfer
Petroleum Based Economy
Biomass Supply
Pretreatment
Sugar platform
Fermentation
Energy & Chemicals
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Bioethanol
Biobutanol
Biodiesel
Biooil
Biogas
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Chemicals
Plastic
Solvent
Drug
Intermediate
Paint
Detergent
Ink
Drink
Cosmetic
• Electricity
• Heat
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3. Development of Bio-ethanol using the Sugarcane Bagasse
Bioenergy
 Bioenergy from different biomasses
 Global Clean Energy Market
Biodiesel
Cellulosic ethanol
Corn ethanol
Current ethanol
Source : National Renewable Energy Laboratory
Source : Clean Edge 2008
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3. Development of Bio-ethanol using the Sugarcane Bagasse
The Economics of Cellulosic Bioethanol
Industrialized Bioethanol
- Brazil: Sugarcane ($1.0/gal)
- USA: Corn ($1.5/gal)
Cellulosic Bioethanol
- $2.43/gal in 2007
- $1.33/gal in 2012
 Production Costs(USD/gallon
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3. Development of Bio-ethanol using the Sugarcane Bagasse
Bioenergy Production from Cellulosic Biomass
Pretreatment
Cellulosic Biomass
Saccharify
Ferment
Bioethanol
Crush
Pyrolysis
Future
Purify
Bio-oil
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3. Development of Bio-ethanol using the Sugarcane Bagasse
Sugarcane Bagasse in Brazil
 The amount of dry bagasse annually produced by the ethanol
and sugar industry is 64 million tons
 Refineries make 4 billion gallons of alcohol fuel
 Ethanol production replaces 460 million barrels of oil
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3. Development of Bio-ethanol using the Sugarcane Bagasse
Composition of Sugarcane Bagasse
Corn stover
Glucan
(%)
36.1
Xylan
(%)
21.4
Lignin
(%)
17.2
Other
(%)
25.3
EFB
47.9
19.1
18.1
14.9
Poplar
42.1
15.1
23.4
19.4
Sugarcane Bagasse
~37.0
~28.0
~21.0
~14.0
Biomass
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3. Development of Bio-ethanol using the Sugarcane Bagasse
Bottleneck in cellulosic ethanol production
Biomass collection
& supply
Cellulase
 Enzyme cost
 Low activity & stability
Enzyme
Crushing
Pretreatment
Detoxification
Biomass recalcitrance
Toxic materials formation
Toxic chemicals use
Recovery of C5 sugar
Saccharification
C6 Ferment.
Purification &
Dehydration
Ethanol
C5 Ferment.
High Cost equip.
& operation
Low ethanol yield
Low ethanol conc.
C5 fermentation
Low saccharification yield
Glucose feedback inhibition
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3. Development of Bio-ethanol using the Sugarcane Bagasse
Pretreatment of Sugarcane Bagasse
Eco-friendly Technology
-Pretreatment using Hot Water
-Pretreatment using Alkali (Sodium hydroxide)
-Recovery of Alkali
Bagasse
Drying/
Crushing
Hot water
(Max. C5 yield)
NaOH
(Max. C6 yield)
Continuous
Process
Pilot Plant
Saccharification
Process
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3. Development of Bio-ethanol using the Sugarcane Bagasse
Enzymatic Saccarification of Pretreated Bagasse
Establishment of Sugar platform
- Cellulase production using fungus and recombinant yeast
- Enzymatic hydrolysis(Sugar yield: 80% above)
Development of high activity Cellulase
Screening of cellulase producing microbes
Cloning of cellulase gene
Expression & Evolution of Cellulase
Evolution by protein engineering
Yeast TFP technology
Recombinant yeast producing
cellulase in g/liter level
Solid state
fermentation
using fungus
Optimize
Sacchari.
Taylor made
cellulase
cocktail
process
Sugar
Platform
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3. Development of Bio-ethanol using the Sugarcane Bagasse
Development of Fermentation Process
SHF
SSF
SSCF
Cellulase
production
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1
1
Hydrolysis
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- Clostridium thermocellum
- 60% cost-down
C6
Hexose
Fermentation
C6
Pentose
Fermentation
C5
 Mascoma’s CBP process
CBP
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C6/C5
C5
SHF: Separate hydrolysis & fermentation
SSF: Simultaneous saccharification & fermentation
SSCF: Simultaneous saccharification & co-fermentation
CBP: Consolidated Bioprocessing
1/4 cost of SSCF
Lynd et al.(2005)
http://greenoptions.com/author/claytonbodiecornell/feed/
- Novel Microbes
- Microbe Engineering
Clostridium
E. coli
Zymomonas
S. cerevisiae
Pichia stipitis
Aspergillus
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3. Development of Bio-ethanol using the Sugarcane Bagasse
Related Technologies Developed in KRIBB
 Penicillium verruculosum COKE4E having cellulase activity (Korean patent application No.
10-2011-0060269)
 Method for the production of biomass having enhanced cellulose content through
sequential acid-/alkali-pretreatment of empty palm fruit bunch (Korean patent application
No. 10-2011-0119175)
 Beta-glucosidase having enhanced activity and method for ethanol production using the
same (Korean patent application No. 10-2011-0034162)
 Novel exo-glucanase and their usages (Korean patent application No. 10-2011-0034402)
 Novel autonomously replicating sequences from thermophilic yeast Kluyveromyces
Marxianus and method for high efficient transformation using the same (Korean patent
application No. 10-2011-0045866)
 Screening of Abundantly Secreted Proteins and Their Use as Fusion Partners for the
Production of Recombinant Proteins (Korean patent application No. 10-2011-7015465)
 Method for Ethanol Production by Simultaneous Saccharification and Fermentation (Korean
Patent No. 050074)
 Method for Preparing Ethanol or Organic Acid Using Recombinant Prokaryote
Overexpressed fabA Gene (Korean patent application No. 10-2009-0032545)
 Microorganism Variants Having Ethanol Producing Ability Using Glycerol or Crude Glycerol
and Method for Producing Ethanol Using the Same (Korean patent application No. 1022
2010-0086550)
Name
Proposed Agenda
E-mail
1. Development of Cosmetics &
Life Cares from Biodiversity
[email protected]
+82-42-860-4750
Dr. Kwang-Hee Son
2. Development of Core Technology for
2nd Generation Bio-ethanol
[email protected]
+82-42-860-4553
Dr. Chul Ho Kim
3. Development of Bioethanol
[email protected]
+82-63-570-5110
Dr. Ho Yong Park
using the Sugarcane Bagasse
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We are looking for partners to the global market !!
Korea Research Institute of Bioscience and
Biotechnology (KRIBB) 韓國生命工學硏究院
Muito Obrigado^^
Thank You !! 감사합니다