Transcript  The blood is collected from animal through puncture of jagular vein in horse ,cattle,camel,sheep and goat &from cephalic vein (recurrent tarsal.

 The blood is collected from animal through puncture
of jagular vein in horse ,cattle,camel,sheep and goat
&from cephalic vein (recurrent tarsal vein)in dog and
cat
 And from ear vein →for isolation of blood parasite
with exception of tryponosoma(from J.V)
A-Whole blood sample:
 The blood sample is mixed with anticoagulant such
as:heparine or E.D.T.A
 It is used for hematological examination such
as:R.B.Cs count,W.B.Cs count, differential leukocytic
count,P.C.V,HB% and blood film
B-SERUM SAMPLE:
 The blood sample is collected without addition of anticoagulant
and then left to clot
 Then centrifugated at 3000 r.p.m for 20 minutes
 Take the clear serum and separate it in a clean blastic container
for biochemical examination
***Blood film
a-a drop of fresh blood is placed in one corner end of slide and
spreader as smear with the help of another slide using it is thin
edgr ar an angle of 45(thin blood film)
b-dry the smear in aur
c-fix the smear in methanol for 4-5 minutes or absolute methyl
alohol for 1-2 minutes
d-dry in air
e-stain the smear with gimsa stain diluted (1:10)in distilled water
for 5 minutes
f-wash the slide with water
g-dry in air
h-examine under oil immersion lens
N.B:
*in case of trypanosoma→make a thick blood film
***indication of oblood film:
 For detection of the presence of blood parasite such
as:babesia,thileria,anaplasma,tryponosoma and
filaria
 For differential leukocytic count