Deep Clean

The latest technology in floor cleaning

Introducing…..MULTIKLEEN

For Floor

Deep Cleaning

The cleaner with added benefits

...

“extended microbial cleaning capabilities”

BIOSOL Multikleen is designed specifically for general floor cleaning & industrial kitchen floor degreasing Organics collect in pores grout Standard floor cleaning leaves grease and grime imbedded in surface pores.

Detergents solubilize grease and soils

ADVANTAGE: Dissolved grease and soil are removed as water is removed DISADVANTAGE: Evaporating residual water re-deposits dissolved soils and grease Non-dissolved grease and soils remain in pores Grease and detergent can produce a polymeric coating on surface Multikleen technology.. residual bioenzymatic action to finish the job.

Problem …....

• Imbedded grime discolors grout, collects in cracks and crevices, and harbors unwanted bacteria.

• Collected organics emit unpleasant odors that indicate uncleanliness and encourage insects and pests. • As moisture is spilled, grease is lifted causing slip hazards.

Biochemistry of grease degradation…

Typical grease = triglyceride glycerol backbone (10% of theoretical BOD) plus three fatty acids (palmitic, stearic, oleic) Lipase cleaves glycerol from fatty acids glycerol & short-chain fatty acids degrade but long-chain fatty acids tend to build-up (palmitic, stearic, & oleic acids) Lipase Glycerol

3

Fatty acids

Biological requirement….

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Stable product combining detergents and bioenzymatic activity requires spores.

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However, Bacillus sp. are not known for degradation of exogenous fatty acids.

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Evidence of degradation by some non-spore forming Gram-positive organisms

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Evidence of degradation by Gram-negative strains

Over two years in development…..

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Microbes capable of degrading all components of grease, including long chain fatty acids

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Microbes that act in the conditions of this application - must germinate - must degrade in the presence of the detergent blend

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Microbes stable in a formulation

Result: n ew patent-pending technology..

Superior grease degrading capability based on a new, patent-pending long-chain fatty acid degrader. Blended into a consortia of rapid grease degraders a new “patent pending spore” strain provides the ability to degrade even the most difficult-to-degrade fractions of grease... leaving floors “squeaky clean”.

Multikleen uses activation chemistry to speed germination...

Degradation by FAD is dramatically faster when combined with a special biochemical activator or co metabolite…...

1400 1200 1000 800 600 400 200 0 0 100 200 300 Time, hours 400 500 Stearic Acid Stearic Acid plus Activator Activator

Multikleen Technology combines bioenzymatic action with safe, environmentally friendly chemistry to make a highly effective product…

Many surfactants interfere with germination..

1 0 0 9 0 8 0 7 0 Formula 7 Formula 8 We optimized the formulation both for cleaning PLUS..

6 0 5 0 4 0 3 0 2 0 microbial action. 1 0 0 0 1 0 2 0 3 0 4 0 5 0 6 0 7 0 8 0 9 0 T ime (hrs.) 1 0 0 1 1 0 1 2 0 1 3 0 1 4 0 1 5 0 1 6 0

Multikleen cleans better than standard chemistry upon application..

And Multikleen provides additional benefits..

residual cleaning effects with better technology

Field testing demonstrates…

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effective cleaning

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improved slip resistance

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biocontrol Field testing locations...

TESTING LOCATIONS

• Two in kitchen:

FIELD TESTING LOCATIONS

• One in customer area:

Slip testing….

In-house testing among three operators was performed to develop technique such that our variance was no greater than the variance reported in support of the ASTM method listing.

Sampling frequencies were selected to support detecting a minimum difference of 0.05 in slip index (S.I.) on a scale of 0.0 - 1.0, with 95% confidence.

DESIGN - Slip Testing

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Three time periods were tested:

• • •

pre-Clean Clean @ 3 weeks Clean @ 6 weeks

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On a test date, the selected tile in each area was first tested dry from each tile side (to account for any graininess), then tested wet from each side.

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Twenty-four measurements were made in each area under both wet and dry conditions, resulting in 72 dry, 72 wet, and 144 total measurements.

RESULTS -

Register Cook 1 Cook 2 pre-Clean Clean @ 3 weeks Clean @ 6 weeks pre-Clean Clean @ 3 weeks Clean @ 6 weeks pre-Clean Clean @ 3 weeks Clean @ 6 weeks Dry SI Mean 0.815

0.875

0.865

0.611

0.710

0.707

0.565

0.688

0.644

Wet SI Mean 0.655

0.648

0.662

0.453

0.550

0.524

0.449

0.520

0.540

OSHA standards define SVI lower than 0.5 as hazardous. Regular use of Multikleen eliminated the hazard.

RESULTS Overall bacterial count remains the same regardless of the cleaner used.

HOWEVER- The population changed to predominantly Biological strains that have been demonstrated to be non-hazardous..

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Plate Photo - During Multikleen Use

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DNA Fingerprint comparisons of floor isolates vs. known strains in product to be compared.

Field Testing demonstrated three benefits:

Compared to standard cleaners Multikleen… •

Made the floor visibly cleaner.

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Displaced potentially harmful microbes with microbes demonstrated to be non-hazardous.

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Produced a significant increase in friction, reducing slip hazard for workers.

MULTIKLEEN

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Designed specifically for cleaning general and greasy kitchen floors.

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Novel approach to cleaning that provides double action….

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FAST cleaning with sophisticated, safe chemistry.

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PLUS patent pending microbial technology that keeps on cleaning long after your work is done….

Residual bioenzymatic “after cleaning” effects provides..

• Cleaner floors • Cleaner grout • Reduced slippage by removing grease • Odor Control • Reduction in conditions that invite and harbor pests

Cleaning that doesn’t stop when you put away the mop……...

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With each cleaning billions of tiny “trained” microbial workers are called to action eradicating grime buried deep in cracks, corners, crevices, and the microscopic pores of floor surfaces.

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Each cleaning removes another layer of embedded grime until the entire surface is “deep clean”.

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As the grime is removed so are the associated odors, removing the major cause of insect infestation, leaving the area fresh and clean.

And it improves workplace safety ..

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Many accidents are caused by slippery floors.

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Slippery conditions result when water lifts residual grease from the floor surface.

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Removal of residual grease eliminates the major cause of slippage.

BIOSOL MULTIKLEEN



Safe to use

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Environmentally friendly