Transcript Fermentation Lab

Fermentation Lab
Fermentation Equation
• C6H12O6
• Glucose
2C2H5OH + 2CO2 + 2 ATP
ethanol + carbon dioxide + 2 ATP
Anaerobic vs. Aerobic
• Anaerobic = without oxygen
• C6H12O6
2C2H5OH +2CO2 +2 ATP
Glucose ethanol + carbon dioxide +2 ATP
• Aerobic = with oxygen
Alcohol Fermentation
• Must be airtight (or it will go into an
aerobic respiration)
• By yeast and some
kinds of bacteria
We’re Using Yeast
• Saccharomyces Cerevisae.
•
Note Budding
CO2 Produces Holes in Bread
• Yeast product of carbon dioxide makes
holes as it expands in the bread
Water Bath:
A beaker inside of a beaker
• This keeps the water temperature even
Ideal Growing Conditions
1. moisture
2. a temperature between 10 and 50ºC but
ideally 30-37ºC
3. a food source such as sugar or flour
• WATCH THE THERMOMETER!!!!!
• Keep it in the beaker.
Gas Collection Tubes
• Take readings
from tube
• Holes already in lid
• Invert in warm
water to act as
waste container for
ethanol
Running a little slow…
• Sheldon will give your solution a boost…
• (yeast loading)
Dry Dormant Stage
Sample Data p. 3
minutes
mL CO2
minutes
mL CO2
1
.5
9
2
1.0
10
4.5
3
1.5
11
5.0
4
2.0
12
5.5
5
2.5
13
6.0
6
3.0
14
6.5
7
3.5
15
7.0
8
4.0
16
7.5