Transcript SyncroPatch 384PE - Nanion Technologies

Nanion Introducing:
SyncroPatch 384PE - the PatchEngine.
Superior Ion Channel Drug Screening.
Nanion:
The SURFE2R.
Catch the wave for transporters.
•
The Patchliner.
Because quality does matter.
•
Unlimited experimental freedom
•
Best of all worlds: throughput,
performance and versatility
•
Press one button and walk away:
- 48 cells in one run
In-depth analysis of transporter
11 years of patch clamping and more to come
The SyncroPatch 96.
Get more throughput.
•
protein activity and function
•
screening
Compatible with diverse
•
membrane sources
•
Multiple targets investigated with
one sensor
Ultra-precise impedance
•
Quick evaluation of cells and
•
Novel experimental possibilities
Real time access to beating
Quick experiments and long term
observations
Quick and easy formation of
GUVs
Membrane
The Orbit 16.
Instant bilayer – just add protein.
•
16 parallel bilayers
•
Low noise, high bandwidth
•
The Vesicle Prep Pro.
Liposomes made easy.
•
Measure More
quality
recordings
parameters
•
High throughput and high data
clamp data
measurements
•
•
Fast access to high quality patch
compounds
•
Ligand- and voltage-gated
channels
The Port-a-Patch.
The world’s smallest patch clamp rig.
•
The CardioExcyte 96.
Bump up your safety screening.
Cost-efficient ion channel
•
Temperature control
•
Stable bilayers for ephys
recordings
Smart tools for ion channel and transporter research and screening
Compatible with your existing
amplifier
The PatchEngine –
a Revolutionary Instrument for APC-based Ion Channel Drug Screening
Executive summary
 384 or even 768 cells in parallel (modular approach)
 Up to 20,000 data points per day
 Reliable data from diverse ion channels targets
 Integrates easily into an HTS environment
 Cost per data point: € 0.2
 Available for shipping NOW!
The PatchEngine –
a Revolutionary Instrument for APC-based Ion Channel Drug Screening
Key features
 384 or 768 cells in parallel (384 or 768 amplifier)
 384 pipettor head
 85 % success rates routinely obtained
 Excellent results with voltage-gated & ligand-gated
ion channels
 Modular approach – easy upgrade & integration
 Single- and multi-hole recording substrates
The PatchEngine – modular, compact and accessible
96
48
Higher throughput
16
Patch Clamping
384
 Stand alone machines
The PatchEngine –
the core of the SyncroPatch
384PE
 Integration
in HTS?
8
The PatchEngine – 100 % integration in HTS environment:
 Fits into commercially available
liquid handler
 Up to two PE’s per robot
 Open design allows integrations
into fully robotic environments
 Used successfully with
Beckman Coulter’s Biomek
The PatchEngine –
the core of the SyncroPatch 384PE
SyncroPatch 384PE – Set-up overview using the Biomek FX robot
Components:
 Biomek FX with a 384 pipetting head
including gripper
 1-2 SyncroPatch 384PE Modules
 1 - 2 x 384 channel amplifier
 SyncroPatch 384 software
 NPC-384 borosilicate chip
 Temperature controlled cell hotel
 Integrated Barcode scanner
 Optional 8 channel pipetting head
(Screen 100% of all compounds)
Open design allows for seamless integration into robotic HTS-highways
Integration of the SyncroPatch 384PE into HTS environment
PlateHotel
FluorescenceReader
Multidrop
Cytomat Incubator
PlateWasher
PlateHandler
SealRemover
PlateSealer
TipLoader
SP 384PE Module 1
SP 384PE Module 2
Shuttle
ThermoShaker
Integration of the SyncroPatch 384PE into HTS environment
HTS Software
Scheduling
Software integration of the SyncroPatch 384PE into the Biomek
Software
Overview Experiment Window
Wellplate overview
Experiment
statistics
One well highlighted
CHO-hKv1.3 cells were
kindly provided by Evotec
Close up
selected traces
Data Control 384 – Outstanding data handling and export
DataControl 384 – intuitive & easy-to-use featuring:
 Intuitive, quick & easy-to-use setup of exps
 Powerful data analysis functions
 Compound view
 Automated data export
 PDF-reporting
Raw data traces of 384 hKv1.3 current responses
SyncroPatch 384 PE – Assay compatibility examples
The SyncroPatch 384 PE has been validated with:
•
•
CHO, HEK, LTK and RBL-1 cells
A variety of voltage-gated ion channels
including hNav1.7, hKv1.3, Kir2.1
•
A variety of ligand-gated ion channels
including including hGABAA α1,β2,γ2 , hGlycine α1
SyncroPatch 384 PE – Consumables NPC-384
Available as single and multi-hole borosilicate plates:
Single-hole substrate:
Four-hole
substrate:
Current Voltage Relationship of hKv1.3 expressed in CHO cells
CHO-hKv1.3 cells were
kindly provided by Evotec
Current Voltage Relationship of hKv1.3 expressed in CHO cells
CHO-hKv1.3 cells were
kindly provided by Evotec
hKv1.3 block by Quinidine
CHO-hKv1.3 cells were
kindly provided by Evotec
hKv1.3 block by Quinidine (5 µM, 50 µM and 500 µM )
Exp length: 16 minutes
Success rate: 85.2 %
Quinidine IC50: 18.9µM
CHO-hKv1.3 cells were
kindly provided by Evotec
Stability of the seal resistance during compound application
Four additions took ca
500 seconds
Success rate: 85.2 %
Four applications:
One control wash and subsequently
three concentrations
Current Voltage Relationship of hNav1.7 expressed in CHO cells
CHO-hNav1.7 cells were
kindly provided by Anaxon
Current Voltage Relationship of hNav1.7 expressed in CHO cells
CHO-hNav1.7 cells were
kindly provided by Anaxon
hNav1.7 block by Tetracaine (5 µM, 50 µM and 500 µM )
CHO-hNav1.7 cells were
kindly provided by Anaxon
hNav1.7 block by Tetracaine (5 µM, 50 µM and 500 µM)
Exp length: 15 minutes
Success rate: 86.2 %
Tetracaine IC50: 49.6µM
CHO-hNav1.7 cells were
kindly provided by Anaxon
Fluidic Properties tested on RBL cells
Exp length: 15 minutes
Success rate: 71,1 %
Exchange rate: <50 ms
Potassium currents through
endogenous Kir 2.1channels in RBL cells
hGABAA α1,β2,γ2 expressed in HEK cells
Three GABA-applications (5 µM) using multi-hole chip
Dose Response Curve of Glycine on the human α1-Glycine Receptor
LTK cells expressing human 1-glycine receptor
Glycine (100 µM, 300 µM, 1 mM) was applied using a stacked
application approach
A multi-hole chip was used.
Cells were kindly provided by AstraZeneca.
SyncroPatch 384 PE – quick priming and quick experiments
Experiment overview
Chip load &
1-2 control washes +
Exp end &
Priming
3 cmpd concentrations
Chip unload
5 minutes
8 – 12 minutes
Total experiment length for 4 – 5 additions: 15
Experiment length is exposure time dependent.
2 minutes
– 20 minutes
SyncroPatch 384 PE – Assay compatibility examples
The SyncroPatch 384 PE assay validation:
Wrap up data section
•
Assay development straightforward, directly adaptable from
other platforms
•
Whole-cell parameters are stable throughout the experiment
•
Success rates for completed experiments are routinely ~ 85%
•
Compatible with voltage- and ligand-gated ion channels
Nanion Technologies – 10 years ago introducing the Port-a-Patch
Reducing a set-up that takes up an entire
room to something so small that you can hold
in your hand.
The Port-a-Patch
 Makes patch clamp accessible to non-experts
 Increases throughput 5 – 10 x
 Allows quick tests of compounds and cells
Nanion Technologies – still increasing the throughput & reducing size
Each PatchEngine:
 384 cells in parallel
 2 units possible
 Integrates easily in HTS
SyncroPatch 384PE – Superior Ion Channel Drug Screening
 Giga-seal recordings
 384 or even 768 simultaneous whole-cell recordings
 Multiple units per liquid handler
 High success rates - 85 %
 Up to 20,000 data points per day
 Voltage- and ligand-gated ion channels
 Competitive price per data point ~ €0.20
 Customized hole sizes and multi-hole chips
… Customized to
your HTS environment
Nanion Customer Reference
- a global customer base distributed between industrial and academic laboratories
Companies:
• Abbott
• Almirall Prodesfarma
• AstraZeneca
• Amgen
• Aurigene Discovery Technologies,
Ltd.
• Axiogenesis
• Bayer
• Bionomics
• Boehringer Ingelheim
•
• Bristol-Myers Squibb
Center for New Drug Safety
• Bionomics
Evaluation and
Research (NCDSER),
• Boehringer
Ingelheim
Chromocell Squibb
• Bristol-Myers
conoGenetix
biosciences
GmbH
• Center
for New
Drug Safety
• Evaluation
Evotec
and Research (NCDSER),
Genionics
• Chromocell
GlaxoSmithKline
• conoGenetix
biosciences GmbH
Grünenthal
• Evotec
• Hofman
La Roche
Genionics
• Hydra
Biosciences
GlaxoSmithKline
Japanese Tobacco
• Grünenthal
Lupin Limited
• Hofman
La Roche
Merck Biosciences
• Hydra
Merch Serano
• Japanese
Tobacco
Novartis
• Lupin
Limited
Novo Nordisk
• Merck
Nycomed
• Merch
Serano
Ono Pharmaceutical
• Novartis
• Orion
Pharma
Novo Nordisk
• Pfizer
Nycomed
PierrePharmaceutical
Fabre
• Ono
Regeneron
Pharmaceuticals
• Orion
Pharma
Rytmion
• Pfizer
Sanofi-Aventis
• Pierre
Fabre
Shinogi
• Regeneron
Pharmaceuticals
Siena Biotech
• Rytmion
SK Holding, Co Ltd
• Sanofi-Aventis
Sundia MediTech Company, Ltd.
• Shinogi
• Suven
Life Sciences Ltd, Hyderabad,
Siena Biotech
• India
SK Holding, Co Ltd
Syngenta
• Sundia
MediTech Company, Ltd.
UCB Pharma
• Suven
Life Sciences Ltd, Hyderabad,
• India
Vertex Pharmaceuticals
Vimta Labs Life Sciences,
• Syngenta
Hyderabad,
• UCB
PharmaIndia
Wyeth Pharmaceuticals
• Vertex
XentionLabs Life Sciences,
• Vimta
Hyderabad, India
• Wyeth
• Xention
Bayer
Research institues and universites:
• Cambridge University, Cambridge, UK
• Charité - Universitätsmedizin Berlin,
Germany
• Consiglio Nazionale delle Ricerche,
Povo (Trento), Italy
• Copenhagen University, Denmark
• Cornell University, Geneva, NY, USA
• Eberhard Karls Universität Tübingen,
Germany
• FMP, Berlin, Germany
• Forschungszentrum Caesar, Bonn,
Germany
• Forschungszentrum Jülich GmbH, Jülich,
Germany
• Friedrich-Schiller-Universität Jena,
Germany
• Georg-August-Universität Göttingen,
Germany
• Griffith University, Nathan, Australia
• GSF - Forschungszentrum für Umwelt
und Gesundheit, GmbH, Neuherberg,
Germany
• Hasselt University, Diepenbeek ,Belgium
• Hertie Institute for Clinical Brain
Research, Germany
• Howard Florey Institute, USA
• Howard Hughes Medical Institute,
Ashburn, VA, USA
• Institute of Genomics and Integrative
Biology, Delhi, India
• IPBS-CNRS, Tolouse, France
• Istituto Italiano di Tecnologia, Genova,
Italy
• Jacobs University, Bremen, Germany
• JNCASR, Bangalore, India
• Julius von Sachs Institut, Würzburg,
Germany
• Karolinska Institute, Stockholm, Sweden
• Kiserleti Orvostudomanyi Kutatointezet,
Budapest, Hungary
• Korea Institute of Science and
Technology (KIST), Seoul, South Korea
• Laboratorio Especial de Toxinologia
Aplicada CAT-CEPID, Sao Paolo, Brazil
• Ludwig Maximillian Universität,
München, Germany
• Lund University, Lund, Sweden
• Max Planck Institut, Göttingen,
Germany
• Medizinische Hochschule Hannover,
Germany
Research institues and universites: (cont.)
• Nanjing University, Nanjing, China
• Nanyang Technological University,
Singapore
• National University of Singapore,
Singapore
• National Center for New Drug Safety
Evaluation and Research (NCDSER),
Shanghai, China
• National Heart Center, Singapore
• National Jewish Medical and Research
Center, Denver, CO, USA
• NIH/NINDS, Bethesda, MD, USA
• NIST, Gaithersburg, MD, USA
• New York University, NY, USA
• RMIT University, Bundoora, Australia
• Ruhr-Universität Bochum, Germany
• Shanghai Institute of Materia Medica,
Shanghai, China
• Simon Fraser University, Burnaby,
Canada
• Southampton General Hospital,
Southampton, UK
• Stiftung Alfred-Wegener-Institut für
Polar- und Meeresforschung,
Bremerhaven, Germany
• Syracuse Univerity, NY, USA
• Technische Universität Ilmenau,
Germany
• Technische Universität Darmstadt,
Germany
• Technische Universiät München,
Germany
• Uppsala University, Uppsala, Sweden
• UNESCO, Bahía Blanca, Argentina
• Universidade Federal de Sao Paulo,
Brazil
• Università degli Studi di Genova, Italy
• Universitatea Lucian Blaga Din Sibiu,
Romania
• University of California, Los Angeles,
CA, USA
• University of California, San Diego, CA,
USA
• University of Cambridge, UK
• University of Cincinnati, OH, USA
• University of Groningen, the
Netherlands
• University of Leeds, UK
• Universidade de Lisboa, Portugal
Research institues and universites: (cont.)
• University of Michigan, Ann Arbor, MI,
USA
• Università degli Studi di Napoli Federico II, Napoli, Italy
• University of North Carolina, Wilmington,
NC, USA
• University of Queensland, Brisbane,
Australia
• University of Rochester, NY, USA
• University of Tennessee Health Science
Center, USA
• University of Valladolid and Spanish
National Research Council (CSIC),
Valladolid, Spain
• University of Washington, USA
• University of Wisconsin, Madison, WI,
USA
• Universität Zürich, Switzerland
• Universitatea Lucian Blaga din Sibiu,
Romania
• Universität Augsburg, Germany
• Universität Freiburg, Germany
• Universität für Bodenkultur, Wien, Austria
• Universität Heidelberg, Germany
• Universität Hohenheim, Germany
• Universität Osnabrück, Germany
• Vanderbilt Medical School, Nashwille,
TN, USA
• Virginia State University, Blacksburg, VA,
USA
• X-Lab, Göttingen, Germany
• Yale University, New Haven, CT, USA
• Inje University, Busan, South Korea