Transcript 72x36 Poster Template - University of Central Florida

PHOSPHATIDIC ACID INCREASES LEAN BODY MASS AND STRENGTH IN RESISTANCE TRAINED MEN
David R. Williams1, Adam J. Wells1, Jay R. Hoffman1, Jeffrey R. Stout1, M.S. Fragala1, G.T. Mangine1, A.M. Gonzalez1, N.S. Emerson1, W.P. McCormack1, T.C. Scanlon1, Ralf Jäger2, Martin Purpura2
1Human Performance Laboratory, University of Central Florida, Orlando, FL, 2Increnovo LLC, 2138 E. Lafayette Pl, Milwaukee, WI 53202
ABSTRACT
Phosphatidic Acid (PA) is a natural phospholipid compound derived from
lecithin which is commonly found in egg yolk, grains, fish, soybeans, peanuts
and yeast. It has been suggested that PA is involved in several intracellular
processes associated with muscle hypertrophy. Specifically, PA has been
reported to activate protein synthesis through the mammalian target of
rapamycin (mTOR) signaling pathway and thereby may enhance the anabolic
effects of resistance training. To our knowledge, no one has examined the
effect of PA supplementation in humans while undergoing a progressive
resistance training program. PURPOSE: To examine the effect of PA
supplementation on lean soft tissue mass (LM) and strength after 8 weeks of
resistance training. METHODS: Fourteen resistance-trained men (mean ±
SD; age 22.7 ± 3.3 yrs; height: 1.78 ± 0.10m; weight: 89.3 ± 16.3 kg)
volunteered to participate in this randomized, double-blind, placebocontrolled, repeated measures study. The participants were assigned to a PA
group (750mg/day; Mediator®, Chemi Nutra, MN, n=7) or placebo group (PL;
rice flower; n=7), delivered in capsule form that was identical in size, shape
and color. Participants were tested for 1RM strength in the bench press (BP)
and squat (SQ) exercise. LM was measured using dual-energy X-ray
absorptiometry. After base line testing, the participants began supplementing
PA or PL for 8 weeks during a progressive resistance training program
intended for muscular hypertrophy. Data was analyzed using magnitudebased inferences on mean changes for BP, SQ and LM. Furthermore, the
magnitudes of the inter-relationships between changes in total training
volume and LM were interpreted using Pearson correlation coefficients,
which had uncertainty (90% confidence limits) of approximately +0.25.
RESULTS: In the PA group, the relationship between changes in training
volume and LM was large (r=0.69, +0.27; 90%CL), however, in the PL group
the relationship was small (r=0.21, +0.44; 90%CL). Changes in strength and
LM in PA and PL groups, and qualitative inferences about the effects are
presented in the table below. CONCLUSION: PA supplementation was
determined to be likely beneficial at improving SQ and LM over PL by 26%
and 64%, respectively. The strong relationship between changes in total
training volume and LM in the PA group suggest that greater training volume
most likely lead to the greater changes in LM, however, no such relationship
was found with PL group. For the BP data, the PA group resulted in a 42%
greater increase in strength over PL, although the effect was considered
unclear. PRACTICAL APPLICATION: While more research is needed to
elucidate mechanism of action; the current findings suggest that in
experienced resistance trained men supplementing 750mg PA per day for 8
weeks may likely benefit greater changes in muscle mass and strength
compared with resistance training only.
INTRODUCTION
• Phosphatidic Acid (PA) is a natural phospholipid compound
derived from lecithin which is commonly found in egg yolk,
grains, fish, soybeans, peanuts and yeast.
• It has been suggested that PA is involved in several intracellular
processes associated with muscle hypertrophy.
• Specifically, PA has been reported to activate protein synthesis
through the mammalian target of rapamycin (mTOR) signaling
pathway and thereby may enhance the anabolic effects of
resistance training.
TEMPLATE DESIGN © 2008
www.PosterPresentations.com
INTRODUCTION CONT.
• To our knowledge, no one has examined the effect of PA
supplementation in humans while undergoing a progressive
resistance training program.
PURPOSE
• To examine the effect of PA supplementation on lean soft tissue
mass (LM) and strength after 8 weeks of resistance training.
METHODS
BODY COMPOSITION: LM was measured using dual-energy X-ray
absorptiometry. After base line testing, the participants began
supplementing PA or PL for 8 weeks during a progressive resistance
training program intended for muscular hypertrophy.
DATA ANALYSIS: Data was analyzed using magnitude-based
inferences on mean changes for BP, SQ and LM. Furthermore, the
magnitudes of the inter-relationships between changes in total
training volume and LM were interpreted using Pearson correlation
coefficients, which had uncertainty (90% confidence limits) of
approximately +0.25.
RESULTS CONT.
(CONT.)
RESULTS
Figure 2. Mean changes in bench
press strength by treatment group.
Table 1. 8 week hypertrophy training protocol.
Figure 1. Study protocol.
Eight Week Resistance Training Program
Monday/Thursday
Tuesday/Friday
Exercise
Bench Press*
Sets/Reps (RM)
1,4 x 10 – 12
Incline DB Press
Seated Shoulder
Press*
Upright rows
Lateral raises
Shrugs
Triceps pushdown
Triceps extension
Situps
3 x 10 - 12
1,4 x 10 – 12
3 x 10 - 12
3 x 10 - 12
3 x 10 - 12
3 x 10 - 12
3 x 10 - 12
3 x 25
Exercise
Squats*
Sets/Reps (RM)
1,4 x 10 – 12
Lunge/Front squat
3 x 10 - 12
Leg Curl
3 x 10 - 12
Knee Extension
Calf Raises
Lat Pulldown
Seated Row
EZ Bar Curl
Dumbbell Curls
Situps
3 x 10 - 12
3 x 10 - 12
4 x 10 - 12
4 x 10 - 12
3 x 10 - 12
3 x 10 - 12
3 x 25
RESULTS
SUBJECTS:
Fourteen resistance-trained men (mean ± SD; age 22.7 ± 3.3 yrs;
height: 1.78 ± 0.10m; weight: 89.3 ± 16.3 kg) volunteered to
participate in this randomized, double-blind, placebo-controlled,
repeated measures study. The participants were assigned to a PA
group (750mg/day; Mediator®, Chemi Nutra, MN, n=7) or placebo
group (PL; rice flower; n=7), delivered in capsule form that was
identical in size, shape and color.
1 RM: Participants
were tested for 1RM
strength in the bench
press (BP) and
squat (SQ) exercise.
1RM squat pre-test
Figure 3. Mean changes in total
body lean soft tissue by treatment
group.
Figure 4. Mean changes in leg lean
mass by treatment group.
• In the PA group, the relationship between changes in training
volume and LM was large (r=0.69, +0.27; 90%CL), however, in
the PL group the relationship was small (r=0.21, +0.44; 90%CL).
• Changes in strength and LM in PA and PL groups, and qualitative
inferences about the effects are presented in the table below.
• Table 2 illustrates changes observed in lean mass, as well as
1RM squat and bench press in PA versus PL.
Table 2. Pre and post mean changes between treatment groups.
SUMMARY & CONCLUSIONS
• PA supplementation was determined to be likely beneficial at
improving SQ and LM over PL by 26% and 64%, respectively.
• The strong relationship between changes in total training volume
and LM in the PA group suggest that greater training volume most
likely lead to the greater changes in LM, however, no such
relationship was found with PL group.
• For the BP data, the PA group resulted in a 42% greater increase
in strength over PL, although the effect was considered unclear.
Change in Measure
PA (mean±SD)
LM(Kg)
SQ(Kg)
BP(Kg)
1.7+1.3
17.2+6.7
6.16+6.1
PL (mean±SD)
Difference +90%CL
Qualitative
Inference
0.6+1.5
12.6+6.1
3.6+7.0
1.1; +1.4
4.5; +6.0
2.6; +6.2
Likely Beneficial
Likely Beneficial
Unclear
PRACTICAL APPLICATION
• While more research is needed to elucidate mechanism of action;
the current findings suggest that in experienced resistance
trained men supplementing 750mg PA per day for 8 weeks may
likely benefit greater changes in muscle mass and strength
compared with resistance training only.