Transcript CHMI 2227E Biochemistry I

CHMI 2227E
Biochemistry I
Proteins:
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Tertiary structure
CHMI 2227 - E.R. Gauthier, Ph.D.
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Tertiary structure
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Tertiary structure
Collagen
myoglobin
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Tertiary structure
http://butane.chem.uiuc.edu/cyerkes/chem104A_S07/Lecture_Notes_104/lect28c.html
Interaction forces
Check this one out: http://www.elmhurst.edu/~chm/vchembook/567tertprotein.html
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CHMI 2227 - E.R. Gauthier, Ph.D.
Tertiary structure
A
CHMI 2227 - E.R. Gauthier, Ph.D.
B
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Tertiary structure
Folding motifs - 1
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Tertiary structure
Folding motifs - 2
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Tertiary structure
Protein domains – Pyruvate kinase
Domain 1
Domain 2
Domain 3
CHMI 2227 - E.R. Gauthier, Ph.D.
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Tertiary structure
1. Myoglobin
b-turn
b-turn
Proline
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Tertiary structure
1. Myoglobin
Hydrophilic amino acids: Blue
Hydrophobic amino acids: Yellow
CHMI Cross-sectional
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view
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Tertiary structure
2. Porin – a membrane-bound protein
Hydrophilic amino acids: Blue
Hydrophobic amino acids: Yellow
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Tertiary structure
Chaperones
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Tertiary structure
Protein denaturation
Check this one out: http://www.elmhurst.edu/~chm/vchembook/568denaturation.html
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CHMI 2227 - E.R. Gauthier, Ph.D.
Tertiary structure
Protein denaturation
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Examples of proteins
1. Green fluorescent protein
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Examples of proteins
1. Green fluorescent protein
Light!
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Examples of proteins
1. Green fluorescent protein
Nucleus
CHMI 2227 - E.R. Gauthier, Ph.D.
Golgi
apparatus
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Examples of proteins
1. Green fluorescent protein
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Examples of proteins
2. Prion proteins
Toxic form = PrPsc
Normal form = PrPc
http://en.wikipedia.org/wiki/Image:Prion.gif
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Examples of proteins
2. Prion proteins
Fiber aggregation
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Important web site:
http://www.pdb.org/pdb/home/home.do
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