Transcript ALAT Chapter 9 - AZ Branch AALAS Homepage

Chapter Nine
Facility Equipment
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Facility Equipment
 Specialized equipment = cage racks, floor
scrubbers, autoclaves, vacuum cleaners and
cage washers
a large capital expense in the operating budget
 It is important that animal technicians learn the
appropriate care and use for each piece of
equipment.
 When equipment fails to operate properly, it must
be immediately taken out of use.
The failure can then be reported to the appropriate
manager and the equipment repaired or replaced as
quickly as possible.
Caging
 Depends on nature of research & husbandry
 Safe cages have smooth surfaces with no jagged
edges, broken wire rungs or rust.
 Escape-proof & well ventilated to maintain
environment
 Poorly ventilated cages - water and urine may
soak animals and increase humidity.
 Dirt and waste deposits collect cracks & crevices.
 Proper cage design permits safe handling of
animals & protects animal handlers.
Caging Materials
 Stainless steel
smooth, durable, no rust &
is impervious to descalers
expensive, but durability offsets high price
 Aluminum
weighs less than stainless steel but is less durable
 Galvanized steel, iron, & wood
not suitable caging materials
rust & don’t stand up to most chemical cleaners
wood - rough, splintery surface, difficult to sanitize
sealed w/ epoxy paint is more easily sanitized, but does not
stand up to high wash temps or scrubbing
Plastic Caging Materials
Polystyrene
melts or warps at wash temp, moderate strength
used for single use disposable rodent caging
 Polypropylene
holds up at high wash temperatures but is
opaque
non-transparent may be preferable for solitary
species
 Polycarbonate
high impact strength, transparent, holds up at
high temps
 Plastics do not rust, their surfaces are
smooth, & they are impervious to chemical
cleaners
Shoebox Rodent Cages
 Solid-bottomed plastic or stainless steel cage
 Contact bedding is generally used.
 The water bottle and feeder are easily visible.
 Microisolation systems > control of environment
 Micro-Isolator™ has own filter top to limit air
exchange between room & cage interior.
 May sit on standard rack, or ventilated rack.
 Procedures inside hooded, ventilated workbench.
feeding, watering, cage changing & manipulations
(Images) Microisolator Changing Hood
Micro-Isolator™
Courtesy of Lab Products, Inc.
Suspended Cage
Systems
 Have perforated or solid bottom
 Wastes drop through bottom into collection pan.
routinely emptied and cleaned
 Flooring can injure or irritate animals’ feet & legs.
 Plastic-coated mesh and bars reduce irritation & provide a
warmer, more comfortable surface.
 Smooth metal floors are comfortable alternative.
 May have individual lids, or suspended on runners which
enable shelves to serve as lids.
 Wire mesh cages better ventilation than solid.
 Newborns fall through wire; direct contact w/ metal draws heat
away from animals’ bodies.
Front-Opening & Metabolism Cages
 Front-Opening Cages
Available as individual or multiple-cage rack
Bar or wire mesh flooring w/ collection trays
Feeders & bottle holders attach to cage door.
Rabbits, cats, dogs & primates usually housed in frontopening cages.
 Metabolism cages designed to separate urine &
feces for specimen collection.
frequently & carefully hand cleaned
prevent feed & water from mixing with specimens
powdered feed helps prevent dropping into collection
drinking valve outside cage to avoid contamination of
collected urine sample
Two Types of Mouse Metabolism Cages
Gang Cages
 House groups of same animal species
 Resting boards or perches make better use of
space.
 Bedding on the cage floor depends on species.
 Feed and water offered in >1 location to avoid
food hoarding by dominant animals.
 Cats, primates & sheep
 Introducing/creating
group may result in
fighting.
Make careful
observation of group.
Transport Cages
 Shipping cartons - temporary
transport housing
 Moist mash, fresh fruits or
vegetables can be source of moisture.
 AWA regulates shipment of certain species, such
as guinea pigs, hamsters, rabbits, cats, dogs &
nonhuman primates.
Regulations cover shipping carton construction, space
requirements, documentation, feed & water schedules.
 Transport cages within a facility
Feed, water & bedding usually not required, since
animals only in cage for a short time.
If transporting in public areas, advisable to use
covering.
Runs or Pens
 Runs or pens are used for larger animals.
 Large enclosures usually with waterproof
flooring & often with a resting area elevated
above floor
 Floor slopes to aid drainage of water and urine
away from housing & feeding areas.
 Surfaces should be easy to clean and sanitize.
 When built outdoors, pens & runs must also
include shelter from inclement weather.
 Pens and runs are commonly used to house
dogs, sheep, pigs and goats.
Feeding & Watering Accessories
 Pelleted feed held in slotted V-shaped feeders in
cage lids or in feeders clipped onto cages.
 J-shaped feeders used for smaller sized pelleted
rabbit and guinea pig feed.
 Feed & water bowls on floor used for dogs &
cats.
 Suspended or detachable feeders that keep feed
off the floor help eliminate contamination.
 Water - glass or plastic bottles, stainless steel or
rubber bowls, pails or automatic watering system
 Bottles or bowls to monitor amount consumed
Some types of medications administered in water.
(Image) Watering and Feeding Devices
Automatic Watering
 Eliminates work associated with water bottles.
 Water through piping to animal area, where
pressure is reduced to suitable levels.
 Pressure reducing station
has 1 - 2 pressure regulators
 filters out particulates from the water supply
 Water then passes into room distribution system
to direct water to cage rack
Retractable recoil hoses tie room system with animal
cage rack manifold system.
Designed to pipe water to each cage and provide a direct
source of water via a drinking valve device.
2 Pressure Water Flow
(Images) Automatic
Automatic
Reduction
Station
1
Treatment
Tanks
4
Sipper
Water
Flow
3 Recoil
Hose
Automatic Watering II
 Individual valves must be checked regularly.
 Clogged or leaking valves should be replaced
immediately or identified as nonfunctional.
 Check pressure station gauges daily.
 Flush to reduce accumulation of microorganisms.
 Some facilities use chemicals such as chlorine to
sanitize water systems.
 It may be necessary to provide newly received
animals with water bottles until they learn to use
the automatic watering system.
Rabbits often require several days to adjust.
An animal that is thirsty will not eat.
Cage Wash Equipment
 Cabinet washers - loaded & sanitized in chamber
Washer goes through washing & rinsing cycles followed
by a drying cycle.
 Rack washers are large versions of cabinet
washers - racks of caging directly rolled in.
single door or pass-through
 Tunnel cage washer is like a commercial car wash.
carries equipment through washer on a conveyor belt
series of stations wash, rinse & dry
 Cage wash controls permit temp control, timing of
cycles, detergent addition & emergency shutoff.
also have monitoring and/or recording gauges
(Images) Scenes From Cage Wash
Cage Wash Equipment II
 Cleaning filters & spray valves
ensure that spray valves do
not become plugged.
 Experienced specialists examine & service
motors, gaskets & electrical components.
 Check timers & gauges daily to ensure
equipment is properly sanitized.
 Some facilities use pressurized steam cleaners to
complement initial hand cleaning of cages.
When no cage washers are available, these cleaners
may be primary method for cage sanitation in
conjunction with disinfectants and detergents.
(Images) Cage Wash Equipment
Balances
 Technicians may weigh animals, obtain body
temperatures or take other measurements.
 Capacity of a scale or balance refers to max.
weight device can measure accurately.
 Accuracy of a balance refers to degree
measured weight = actual weight.
 An efficient balance allows small weight
increments to be distinguished with accuracy.
 Speed and ease of operation important in
choosing the balance best suited for a
particular job.
 Top-loading balances are most common.
Balances II
 Triple-beam & double-pan for smaller species.
 Large species platform scales read weight
directly, similar to a home bathroom scale.
Some are designed so animal is restrained.
 Top-loading balances w/ digital readouts.
Pan weight set at zero automatically to eliminate
weight of restrainers before measuring animal.
Some models provide printout.
 Electronic balances can input data to a computer.
 Cleaning scales after each use is important to
prevent transmission of disease & prevent dirt &
hair accumulation from affecting scale operation.
Additional Reading
Poole, Trevor (ed.). The UFAW Handbook on the
Care and Management of Laboratory Animals,
6th. Ed. Longman Scientific and Technical,
Essex, England. 1987
Rollin, Bernard E., and M. Lynne Kesel (ed.). The
Experimental Animal in Biomedical Research.
Volume II: Care, Husbandry and Well-Being —
An Overview By Species. CRC Press, Boca
Raton, FL. 1992