Quick View In-Line rapid strep a testing
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Transcript Quick View In-Line rapid strep a testing
QUICKVUE IN-LINE RAPID
STREP A TESTING
WellOne Primary Medical
and Dental Care
For Medical Clinical Staff
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WARNING
Conducting this test exposes the operator to potentially
infectious material. Standard precautions, including glove
use, are required for this procedure.
this test exposes the operator to potentially infectious
material. Standard precautions, including glove use are
required for this procedure.
Controls are potentially infectious and should be handled in
the same manner as a patient specimen, using all
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appropriate precautions.
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Rapid Strep A Testing: Overview
Pharyngitis, can be caused by streptococci from
serogroups other than Group A, as well as other
pathogens. The rapid strep test will not detect these
other groups and pathogens.
The Quick Vue ® Strep test will not differentiate
asymptomatic carriers of Group A Streptococcus from
those exhibiting streptococcal infection.
The test is designated as a screening test at WellOne
since, if clinical signs and symptoms are not consistent
with test results, follow-up laboratory performed culture
procedure should be performed.
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Question (True or False): A negative Rapid Strep test is
proof positive that the patient does not have strep
throat.
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TRUE
FALSE
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The correct answer is FALSE
The rapid strep test is 92% sensitive (meaning that
8 out of 100 persons with strep throat will have a
negative rapid strep test)
If clinical signs and symptoms are not consistent with
test results, follow-up laboratory performed culture
procedure should be performed.
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Rapid Strep A Testing: Storage and
Stability
Reagents should be stored at room temperature, in the
laboratory 59 F -86F (15 C -30C) and out of direct
sunlight. The laboratory room temperature must be
checked daily and action taken for out of range
temperatures.
Kit contents are stable until the expiration date printed
on the outer box.
Swab specimens should be processed as soon as
possible after collection and the test kit not opened until
just prior to testing.
The Test Cassette must remain sealed in the protective
foil pouch until just prior to use.
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Rapid Strep A Testing: Initial Control
Testing
Each test kit comes with two control swabs (one positive
and one negative) that must be checked prior to use of
a new box of tests
Be sure that controls have been run and are acceptable
for the test kit that you are using prior to patient testing
If results are not as expected (ie. Positive control is
negative and/or negative control is positive) DO NOT
USE BOX. Segregate box of tests and notify supervisor
immediately.
Document the results of control testing on the strep
testing log form
Use a new log form for each new box of tests opened
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Steps in Testing: General
Identify
patient using two patient identifiers (name and
DOB)
Explain procedure to patient in culturally appropriate,
age appropriate terms.
Gather all necessary equipment and supplies prior to
start of the procedure.
Inspect all equipment and supplies for damage, general
integrity and expiration date- do not use any damaged
or expired materials
Perform hand hygiene before and after patient
contact; before and after donning gloves.
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Before conducting a strep test, the
tester must:
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A. Ensure that the lot number on the test
kits begins with 1003
B. External controls have been run on the
test kit and the control results were
acceptable
C. The green lettering on the box has not
faded
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The correct answer is:
External controls have been run on the test kit
and the control results were acceptable.
External controls must be run on each test kit
prior to use and recorded on the strep testing
log.
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Steps in Testing: Collecting the
Specimen
Collect the specimen: Use ONLY the swabs supplied
with the test kit for Strep testing (these swabs have
a green handle)
Avoid the uvula as
stimulation may
result in gagging
Stand off to
the side of the
patient to
collect the
specimen
(helpful if the
patient coughs
or gags)
Take the sample
from both sides
of the throat at
the tonsillar areas
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The specimen should be collected from:
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The uvula
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The posterior gyros
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Both sides of the tonsillar areas
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The correct answer is both sides of the
tonsillar areas
Avoid the uvula as
stimulation may
result in gagging
Take the sample
from both sides
of the throat at
the tonsillar areas
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The proper test swabs to use for Rapid
Strep A testing are:
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Wooden with a cotton tip
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Supplied with the kit and have a green handle
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Supplied with the kit and have a wooden handle
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The correct answer is:
Supplied with the kit and have a green handle
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Strep Testing: Standing Orders for MA
Staff
Collect and test specimen on all patients presenting
with sore throat or patients stating that they have
recently been exposed to strep and are ill.
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Steps in Testing: Testing the sample
Label with patients name (or initials) and date of
birth
Open a test cassette and place on a clean, level
surface.
Insert the swab completely into the swab chamber.
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Steps in Testing: Testing the sample
Inspect the extraction solution bottle and do not use
if the solution is not clear. DO NOT USE
EXTRACTION SOLUTION IF IT IS GREEN PRIOR TO
BREAKING AMPULE.
Squeeze carefully to crush the glass ampule inside
the extraction solution bottle.
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Steps in Testing: Testing the sample
Vigorously shake the bottle 5 times to mix solution.
Solution should turn green after the ampule is
broken (do not use if green color is not present).
Solution must be used immediately.
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The extraction solution bottle should be
crushed and __________ prior to use.
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shaken vigorously 5 times
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warmed
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cooled
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frozen
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The correct answer is shaken 5 times
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The extraction solution before crushing
should:
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Be clear green
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Be pink
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Contain a green hue
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Be Clear
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The correct answer is be clear…..
GREEN before
crushing, the ampule has
already been crushed before
you handled it.
If the solution is
Since the solution must be used immediately after
mixing, the extraction bottle must be discarded.
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The extraction solution (after crushing)
should:
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Not be used if it is not clear green
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Be pink
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Contain a black hue
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Be orange
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The correct answer is clear and green
Before crushing and mixing the ampule, the extraction
solution should be
After crushing and mixing the ampule, the extraction
solution should be clear (not cloudy) and
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Steps in Testing: Testing the sample
Remove the cap from the bottle and quickly fill the
chamber to the rim.
Begin timing (5 minutes)
If liquid has not moved across the Result Window in
1 minute, completely remove the swab and re-insert.
If liquid still does not move across, retest with a new
specimen, Test Cassette and Extraction Solution
Bottle.
The Test Cassette should not be moved until the assay is
complete.
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Steps in Testing: Testing the sample
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Steps in Testing: Interpreting Results
Positive Result:
The appearance of any pink - to purple line next to the letter
“T” in the Result window, along with the blue control Line
next to the letter “C” means that the test is positive for
group A Streptococcus.
C for control
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Even a light line at the T is positive
T for Test
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Steps in Testing: Interpreting Results
The Control line is vivid blueindicating an acceptable test
The Test line is
light pink
indicating a
positive test
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Steps in Testing: Interpreting Results
Negative Result:
The appearance of only the blue control Line next to
letter “C” in the Result Window means that the test
is negative. A negative QuickVue ® result means
that the swab is presumptive negative for group A
Streptococcus. A line at the C= acceptable test
No line at the T= negative test
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Steps in Testing: Interpreting Results
No line at the C=
Test is not
acceptableDiscard and
repeat
If second test does
show a control line,
place the box out
of use and notify
supervisor
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A blue control line next to the letter C
means that:
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The test is positive
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This was a “good” (or reliable) test
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The patient has strep throat
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The results are not valid
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The correct answer is: This was a “good” (or
reliable) test
For more on controls, place mouse here
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Any shade of a light pink to purple line
at the “T” means:
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The test is positive
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This was a “good” (or reliable) test
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The patient has mono
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The results are not valid
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The correct answer is:
The test is positive
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Steps in Testing: Interpreting Results
A negative background control is provided by the
clearing of background color in the result window
and indicates that there were no immunological
interfering substances in the specimen. This area
should be white to light pink within 5 minutes.
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Rapid strep results should be read at:
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20 minutes
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5 minutes
8 minutes
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Steps in Testing: Testing the sample
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You have reached the end of this
program…..
Click below to view a video on
QuickVue Rapid A Strep Testing
http://www.quidel.com/assets/swf/StrepATrainingvideoHQ.htm
Once you are confident that you are comfortable with all the
materials presented, proceed to the QuickVue Rapid A Strep
Testing post test at: http://www.classmarker.com/professional/
Start Over
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