Transcript iGEM TEAM EPF

iGEM TEAM
EPF-LAUSANNE
International Genetically
Engineered Machine
OUR TEAM & OUR SCHOOL
Carine
Christian
Tu Nathalie
Nicolas
Gabriela
Nicolas Rafael
10 undergraduate students
6 advisors
Heidi Sebastian
Caroline
Mélanie
Specific Aims:
1. To develop strategies to regulate gene expression using light as
a stimulus.
2. To use computational tools to optimize the selected strategies.
Our approach:
To utilize defined molecular systems involved in gene regulation.
The activity of the molecules involved should be amenable to
regulation by light-induced changes in protein conformation.
Gene Regulation by Modulating Protein Conformational
Changes:
The LovTAP system
Features:
- Light-activated
- Reversible
- Synthetic system
Gene Regulation by Modulating Protein Conformational
Changes:
Experimental Design
RO#1
RO#2
Experimental Design: iGEM TOOLS
Experimental Design: Cloning Strategy
Results: Read-out n°1 characterization
RO#1 without Tryptophan
RO#1 with Tryptopan
Results: Entire system (LovTAP + read-out n°2)
Specific Aim 2 : To use computational tools to optimize
the selected strategies
The LovTAP system
Desired Features:
• Increased lifetime of the light-activated protein
• Enhanced affinity of the binding of the light-activated protein to the
DNA
Experimental Design: Insight from Molecular
Dynamics and Biochemical Modeling
Applications


Specific and temporal induction of gene
transcription in mammalian cells
Coupling with gene recombination systems
Acknowledgements
Sebastian Maerkl : Laboratory of Biological Network Characterization
 Bart Deplancke : Laboratory of Systems Biology and Genetics
 Matteo Dal Peraro : Laboratory for Biomolecular Modeling
 Felix Naef : Computational systems biology

Thank You For Your Attention