Transcript Document

Štruktúrne a funkčné aspekty
vplyvu kadmia na rastliny
Beáta Bočová (Široká)
Katarína Ďurčeková
Sylvia Budíková
Jana Huttová
Igor Mistrík
Peter Paľove-Balang
Marta Ollé (Šimonovičová)
Ladislav Tamás
Margita Vašková
VEGA 2/4040
[2004-2006]
Effect of cadmium on hydrolytic enzymes in maize root and coleoptile
Beáta Široká, Jana Huttová, Ladislav Tamás, Marta Šimonovičová,  Igor Mistrík
Biologia, Bratislava, 59/4: 513-517, 2004.
300
120
250
200
150
100
80
60
100
40
50
20
0
0
Root tip
Rest of root Coleoptile
Root tip
Rest of root Coleoptile
180
160
140
120
100
80
60
40
20
0
% of control
140
% of control
350
AlPh
AcPh
Est
% of control
% of control
Glu
Root tip
Rest of root Coleoptile
180
160
140
120
100
80
60
40
20
0





A
B
C
Glucosidase (Glu), esterase (Est), acid phosphatase (AcPh) and alkaline phosphatase (AlPh) activity in apical part of roots
(A), basal part of roots (B) and coleoptiles (C) after 24 h exposure of maize seedlings to 1, 10, 50, 250, and 1000 M Cd.
Means  SD of five experiments.
Peroxidase mediated hydrogen peroxide production in barley roots grown
under stress conditions
Marta Šimonovičová*, Jana Huttová, Igor Mistrík, Beáta Široká, Ladislav Tamás
Plant Growth Regulation 44: 267-275, 2004
% of control
900
600
HP production
NADH oxidation
guaiacol-POD activity
300
0
Co
Al
Cu
Cd
Production of H2O2, oxidation of NADH and guaiacol peroxidase activity after 72 h (C) from the
onset of imbibition of control seedlings and seedlings exposed to Co, Al, Cu, Cd. Mean values are
the average of three replicates  S.D. Values followed by the same letter are not significantly
different (p<0.05) according to one-way ANOVA test.
Effect of cadmium on oxalate oxidase activity in barley roots
Marta Šimonovičová, Beáta Bočová, Jana Huttová, Igor Mistrík, Ladislav Tamás
Biologia, Bratislava, 60/4: 463-466, 2005.
[% of control]
250
0
1
2
0
1
2
mM
200
150
100
50
0
48
72
hr
Activity of oxalate oxidase (OXO) barley roots 48 and 72 h after the onset of imbibition
in the presence of 0, 1 and 2 mM Cd. Data represent the means  SD (n  5).
Cadmium induced changes in cell wall peroxidase isozyme pattern in barley
root tips
Jana Huttová, Igor Mistrík, Marta Ollé-Šimonovičová, Ladislav Tamás
Plant Soil Environ 52(6): 250-253, 2006
Anodic and cathodic PAGE of peroxidase activity in CW fractions of barley
roots 72 h after the onset of imbibition in the presence of 0, 1 and 2 mM Cd.
Arrowheads indicate induced, and stars reduced isoperoxidases.
Short- and long-term effects of cadmium on transmembrane electric potential
(Em) in maize roots
Ján Pavlovkin, Miroslava Luxová, Inrid Mistríková & Igor Mistrík
Biologia, Bratislava 61(1): 109-114, 2006
Membrane potential (-mV)
140
120
100
80
60
40
control
Cd
20
0
0
2
4
8
12
16
20
24
[h]
Time dependent changes in plasma membrane potential of outer cortical cells of
intact maize roots exposed to 0,1 mM Cd
Effect of manganase on cadmium toxicity in maize seedlings
P.Paľové-Balang, A. Kisová, J. Pavlovkin, Igor Mistrík
root increment (cm d -1)
Plant Soil Environ 52(4): 143-149, 2006
4,0
3,5
3,0
2,5
2,0
1,5
1,0
0,5
0,0
control
0
10
100
150
200
Mn (μM)
Effect of Cd and Mn ions on root growth of three-day-old maize seedlings. Root
solution contained 0.5 μM CaSO4 (control – open column) supplemented with 10
μM CdCl2 and different concentrations of MnCl2 (0-200 μM – filled columns).
The root growth increments are expressed in cm per day. The results are the
means (+SE) of 4 experiments (n = 20).
Cadmium-induced microsomal membrane-bound peroxidases mediated
hydrogen peroxide production in barley roots
Ladislav Tamás, Jana Huttová, Igor Mistrík and Marta Ollé
Acta Physiologie Plantarum 28: 453-457, 2006
H2O2 production
0,0
0,1
0,2
0,3
0,4
A510 nm
Anodic and cathodic isoperoxidases (stained with AEC and guaiacol) detected 72 h after
the onset of imbibition in the presence of 0, 1 and 2 mM Cd (the Cd-induced peroxidases
are indicated by arrow) and the production of H2O2 in individual gel cuttings of 2 mM Cd
line. Values are the mean  SE (n = 5).
Cadmium-induced inhibition of apoplastic ascorbate oxidase in barley roots
Ladislav Tamás, Beáta Bočová, Jana Huttová, Igor Mistrík and Marta Ollé
Plant Growth Regulation 48: 41-49, 2006
Ascorbate oxidase activity [A265 nm]
1,8
1,5
a
a
1,2
b
cb
0,9
blank
d
d
0.0 mM Cd
0,6
0.25 mM Cd
e
e
0.5 mM Cd
0,3
1.0 mM Cd
0,0
0
5
10
15
20
25
30
Time [min]
Time-lapse activity of ascorbate oxidase (AO; expressed as
decrease in absorbance at 265 due to ascorbate oxidation) in
cell wall (CW) II fraction of barley roots after 72 h of seedtreatment with 0.0, 0.25, 0.5 and 1.0 mM Cd.
Anodic and cathodic PAGE of ascorbate oxidase activity in extracellular (ExC),
soluble (Sol), cell wall (CW I, II, III and IV)- and membrane-bound (MB)
fractions of barley roots after 72 h of seed-treatment with 0.0, 1.0 and
2.0 mM Cd.
Rhizosphere localized cationic peroxidase from barley roots is strongly activated
by cadmium and correlated with root growth inhibition
Ladislav Tamás, Katarína Ďurčeková, Ľubica Halušková, Jana Huttová, Igor Mistrík and Marta Ollé
Chemosphere 66: 1292-1300, 2007
0-2
2–4
4–6
6–8
Distance from root tip (mm)
8 – 10
Cathodic isoperoxidases in extracellular fractions isolated with
40 mM Na - succinate buffer, pH 4.0; containing 1 M NaCl
from 2 mm long root sections after 72 h of seed-treatment with
1.0 mM of Cd. The Cd-induced isoperoxidase is indicated by
arrow.
Cadmium induces premature xylogenesis in barley roots
Katarína Ďurčeková, Jana Huttová, Igor Mistrík, Marta Ollé, and Ladislav Tamás
Plant and Soil 290: 61-68, 2007
Effect of Cd on the
spatial distribution of
H2O2 production (A)
and POD activity
using SYR (B) or 4MN (C) as substrate in
barley
root
early
metaxylem 72 h after
the
onset
of
imbibition.
Arrows
indicate the positive
stain for H2O2 and
POD activity. (Ccontrol, Cd-cadmium
treated).