Transcript Calibration and Measurement

Image-Pro Premier
Basic Training Course
Part 4 - Calibration and Measurements
Measuring Objects
Groups of pixels together
make objects
From Outlines we can get
measurements:
Perimeter
Area
Bounding box
Centre coordinates
Radii
Much, much more...
Calibrations
Calibrations make sense of
measurement
Transforms distances from
Pixels to real world units
All images must be
calibrated for spatial
measurements
40µm
Creating Calibrations
Use known values to
estimate
•Cal
= Sampling Rate
Instrument Mag
Or, take image of reference
object
Creating Calibration
1. Take image of known sample
2. Go to Capture Ribbon
3. Select Quick Calibration tool
4. Drag line over known distance
5. Select units and qualify line
Calibrating Images
From Capture Ribbon
Select Calibration from
Characteristics box
New automatically assume
current charactristics calibrations
Use green tick mark next to
Spatial drop-down for image with
no or incorrect calibration
Scale Bars
Calibrations enable Scale Bars
and Measurements
Enable Scale Bars from
Calibration group
Use Auto Marker to apply
markers to every image
Use Calibration drop-down to
access Marker properties
1mm
Manual Measurements
Use Measure ribbon for
manual measurements
Use Types button to select
what values to collect
Use drop-downs for
additional tools (e.g.
straight line Vs poly-line)
Display Option
Use Options button to
change:
Outline styles
Label option
Display Option
Show statistics in
Measurement table
Use groupings to
arrange object data by
class
Change grouping
options to display class
statistics
Exporting Data
Export Images using:
Quick Save in
Capture ribbon
Share ribbon
Export any data table
to Excel, Text files or
Clipboard
Measurements
Manual Measurements are a powerful tool for
getting information from an image but...
...Manual measurements are also:
slow & tedious
open to operator bias