Transcript Cytogenetic practicle

Q1. Ten blood samples are set up, cultured by routine
methods. All Showed low Mitotic Index, EXPLAIN?
briefly!
Q2.Choose the most correct answer(s)
A. Fixed pellets of patients with an abnormal karyotype should be
1)kept for 6 months at least
2) kept indefinitely
3) kept until the final report is issued
4) 1 and 2
B. Fetal calf serum
1) Contains agents which stimulate red cells to divide
2) Helps to buffer the culture medium
3) Provides essential nutrients for tissue culture
4) Prevents blood coagulation in blood cultures
C-Which best describes the action of Colcemid in a harvest:
1)Inhibiting centriole activity 2) Inhibiting spindle fiber formation
3) Swelling the cell
4) Inducing chromatin condensation
D- A gamete contains:
1) 2n=46 2) 2n=23 3) n=46
4) n=23
E- It is safe to harvest blood in a Biological laminar flow cabinet:
1) True
2) False
F- The risk of Blood culture contamination is high:
1) True
2) False
G. Na Heparin
1) Is a blood clotting agent
2) Binds calcium and magnesium ions
3) Binds chloride and fluoride ions
4) Is a B lymphocyte mitogen
H. A CO2 Incubator is used in an open tissue culture system because
1)CO2 is bacteriocidal
2) CO2 is an essential cell nutrient
3) The presence of CO2 reduces oxygen tension
4) CO2 is part of the culture pH buffering system
I. GTG banding refers to the process in which G-banding is
treated chromosomeswith ---------------. G-banding stains the
regions of DNA that are rich in --------&-------, While the
regions of chromosome that are rich in ----------&-----------have little affinity for the dye and remain light:
1)Protease, A&T, G & A
2)Trypsin, A&T, G&C
3)Trypsin, G&C, A&T
4)Trypsin, G&A, C&T.
J. Increasing acetic acid concentration in fixative is likely
to increase metaphase spreading:
1) True
2)False
K. The hypotonic solution used in short term Blood
harvests is:
1) 0.06M KCl
2) 0.075M KCL
3) 1%KCL
4) 1% sodium citrate
Q3. The chromosomes in this figure are not in proper place!!
A- Chromosome at no.2 should moved to no.--------------,
B-. Chromosome at no.10 should moved to no.-------------C- Chromosome at no.18 should moved to no.--------------,
D- Chromosome at no.19 should moved to no.--------------,
Q4
Q5: The Human karyotype is------------------------
Q6
B
A
Q7
B
A
D
C
Q8
A
D
B
C
Q9
*Dear Student: Expect your mark out of 30!!!!!
Write your expectation in the answer sheet.
ALL THE BEST
Dr. RAIDA W. KHALIL