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GA remained stable
up to 100 °C while the
yield of VA decreased
at 120
°C.Effect of
Fig. 3.
different extraction
temperature on the
recoveries of (A) GA
and(B) VA by MAE
with extraction time:
20 min.
Fig. 4. Effect of
extraction
time
the
The optimized MAE conditions for VA were
at 100
°C on
and
recoveries of (A) GA
10 min.
and (B) VA by MAE
at extraction
The optimized MAE conditions for GA temperature:
were at 100 °C
100and
◦C.
20 min.
MAE can save a lot of time for the extraction of many
Table 2.
samples.
Comparison of amount of GA and VA extracted from different
batches of G. elata Blume using MAE and heating under reflux
with water as extraction fluid
A longer extraction time would result in lower yield for VA.
The extraction efficiency of MAE was comparatively higher
than heating under reflux of GA at 100 °C for two different
batches of botanicals.
Both methods had demonstrated
to be efficient in the extraction of
GA and VA .
Fig. 5. Chromatograms
obtained for GA and VA
in GE by (A)
PHWE at
Table 1
120 ◦C and (B) MAE at
MAE showed better extraction efficiency
100 ◦C for GA
compared to PHWE
Table 2
Conclusions
 PHWE and MAE showed that thermally labile
compounds could be extracted at an optimized elevated
temperature using pure water as extraction solvent
without the addition of organic modifier/solvent.
Conclusions
 The extraction efficiency of PHWE and MAE was
found to be comparable or higher compared to heating
under reflux.
 PHWE and MAE methods were shown to be feasible
alternatives for the extraction of thermally labile
marker compounds present in medicinal plants.
Thank you for your attention
HPLC
幫浦
層析管柱
注射閥
紫外光
偵測器
移動相儲瓶
廢液
數據處
理系統
記錄器
移動相儲瓶、高壓幫浦、注射閥、層析管柱C18 RP(25 cm×I.D 4.6 mm)或同級品。
紫外光偵測器(使用 270 nm)、數據處理系統或記錄器
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