Transcript Mechanical Characterization of Cancerous Versus Healthy Fibroblast Cells (PPT | 892KB)

Mechanical Characterization of
Cancerous Versus Healthy Fibroblast
Cells
Team Members: Amy Lutkus, Cristofer Madera, Bhanu Peddi
Advisor: Raffaella De Vita, Ph.D., Jeannine Strobl, Ph.D.
The goal of this project is to successfully characterize cancer
cells and fibroblast cells using Atomic Force Microscopy (AFM) to
characterize the actin filaments and overall structure of the cells
through their mechanical properties while providing a tensegrity
model.
Project Overview:
• Investigation of different substrates for optimal
attachment
• Study of the effects of fixation on the mechanical
properties and characterization of the cells
•Conduct Atomic Force Microscope measurements for
analysis of mechanical properties
•Production of efficient MATLAB code for modeling
analysis
•Optical and fluorescence microscope imaging for
understanding cell behavior
•Design and build a reliable and predictable tensegrity
model for future use
•Writing a journal article to present for further research