Transcript Fig. S1

Fig. S1
Lethal Heat Survival Assay
Strains were grown to mid-logarithmic phase (5 x 106 cells per ml) in YEPD medium, washed in PBS, and
concentrated to 2 x 108 cells per ml. Aliquots of 100 μI were prepared in Eppendorf tubes. Then cells
were heated at 50°C in water bath for 60 minutes. Tubes were removed and immediately placed on ice.
The aliquots were then diluted accordingly and plated onto YEPD plates. Colonies were counted after 3
days of incubation at 30°C, and the percent viability was calculated with respect to cells not heated.