Transcript Human Mitochondrial DNA

Assay for an Antibiotic
Resistance Enzyme
Carolina Kit
Timeline
Tuesday—Lecture,Review concepts of
procedure
Thursday—Part 1
Part 2 (volunteer to serve as
demonstrator—4 hours lab service)
Monday--handouts due
Prep. For lab—Assay for Anti-biotic
resistance
2 days prior (Tuesday)
--label tubes
control (8--15mL tubes)
pAMP (8--15 mL tubes)
penicillin-G (8--15 mL tubes)
--streak fresh plate of MM294 and MM294/pAMP (24 hours at 37 incubator)
1 day prior (Wednesday)
--prepare cultures (1/group)—see teacher guide (24 hours at 37 incubator)
--penicillin-G solution (store at -20 until use)
--aliquot (8 groups)
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•
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MM294 5ml overnight culture, label control
MM294/pAMP 5ml overnight culture, label pAMP
5ml of 4mg/mL penicillin-G
On lab day 1 (Thursday)
--test the reagents with phenol red
Lab day 2 (Monday)
--demonstrator of spectrometer
Write-up
• Complete all aspects of the handout (50
points)
Staple these together. Drawings should be
neat!