Transcript Micropipette Lab

Micropipettes
High Range: 100-1000 µL
Digits from top to bottom
represent:
1000’s
100’s
10’s
●
●
This pipette is set to
1000 µL → → → →
The 1’s are indicated by the
tiny white tick marks in the
dial with the 10’s. Marks are
in increments of 2 µL
Use large blue or white tips
This pipette is set to
100 µL
This pipette is set to
990 µL
Mid-range:
5.0-50.0 µL
10.0100.0 µL, 20.0-200.0
µL
● Range is printed on
the button or on the
side
● Use medium sized
yellow or white tips
10.0-100.0 µL
This pipette is set to its
maximum: 100.0 µL
Digits represent:
100’s
10’s
1’s
● Tick marks represent 0.1’s in
increments of 0.2 µL
Low Range:
0.50-10.00 µL
2.00-20.00 µL
Digits represent:
10’s
1’s
0.1’s
● Tick marks are 0.01’s in
increments of 0.02 µL
● Use small white tips
Rainin 0.50-10.00 µL pipettes use
a unique tip
Clear tip has an extra wide mouth
that surrounds and goes over the
barrel and eject bar.
These tips can ONLY be used with
the Rainin 0.5-10
Feel the stops before you begin taking up or dispensing any liquid.
1st stop: Exact volume
to which the pipette is
set. Press to this stop
before immersing
pipette tip in the
desired solution.
2nd stop: Use when
dispensing solution.
Pushes an extra
volume of air to
dislodge any solution
adhering to the tip.
How to pipette
Great summary
•Extract and dispense SLOWLY.
•When dispensing, touch the tip
to the side or bottom of the tube,
or if there is liquid already in the
tube, touch the tip to the liquid.
•When taking up or dispensing
liquid, don’t put the tip any deeper
into the liquid than necessary.
•Never hold pipette so that the tip is higher than the handle or liquid
leaks into the barrel (BAD!)
•Always hold pipettes
vertically when in use
•Never go above MAX volume!
•Check to be sure the tip is on securely.
•Only open the tip box when getting a tip - DON’T LEAVE IT OPEN!
•Don’t set the pipettes past their intended range (high or low), even
though you can.