Transcript Micropipette reminde.. - Baliga Lab at Institute for Systems Biology

Reminders when using the
Micropipette
•
•
•
•
•
•
•
•
•
•
Always keep the micropipette in a vertical position when there is fluid in
the barrel so the liquid will not run back into the barrel.
Set the desired volume before getting a tip. Never attempt to set a
volume beyond the pipette's minimum or maximum values.
Always change tips for each new solution you need to pipette.
Avoid contamination. Never touch a tip and keep tips from touching lab
bench.
The plunger has two stops. The first stop is for drawing up the desired
volume of liquid and the second stop is for completely dispensing the
sample.
Look at the liquid in your tip. If there are air bubbles, try again!
Do not allow the top of the micropipette tip to be completely submerged into
any of the solutions.
After dispensing the sample into its container, keep the plunger depressed
until the tip is completely out of the container.
Do not leave the micropipette standing in a solution to avoid spilling the
solution.
If you are mixing together two different liquids, always add the smallest
volume last.
Institute for Systems Biology & Bellevue School District