IN VITRO CULTURE OF PRIMARY HUMAN PLEURAL MESOTHELIAL CELL LINES

ÖNER DİKENSOY, BÜLENT GÖĞEBAKAN, EROL TÜKENMEZ, HASAN BAYRAM.

GAZİANTEP ÜNİVERSİTESİ, GÖĞÜS HASTALIKLARI AD.

GAZİANTEP [email protected]

Aim-1

• In vitro cell cultures of the respiratory system are commonly used in experimental studies worldwide • Their usage in Turkey is rather limited. • In 2006, an in-vitro cell culture laboratory was established in the Department of Pulmonary Diseases of Gaziantep University • In this lab, mainly studies of airway epithelial cell cultures are conducted.

Aim-2

• Pleural diseases are still important causes of mortality and morbidity in Turkey and Worldwide.

• To the best of our knowledge, primer mesothelial cell culture has not been done in Turkey before.

• The aim of this study was to culture primary human pleural mesothelial cells in the laboratory set up for experimental studies.

Method-1

• Primary pleural mesothelial cells were obtained from a volunteer having transudative pleural effusion by means of thoracentesis, as previously described in the literature.

• Briefly, thoracentesis was performed under sterile conditions using a closed aspiration system. • Approximately 300 mL of transudative effusion that was drained from the patient transferred to the laboratory. • After the fluid was centrifuged at 4000rpm for 5 minutes, the supernatant was aspirated,

Method-2

• Then the remaining pellet was re suspended in ammonium chloride solution for the elimination of erythrocytes by lyses. • The remaining cells were cultured in a cell culture flask in Dulbeco’s Modified Medium containing foetal calf serum, antibiotic and antimycotic solution.

Cell Culture Laboratory

Results

• Cells were adhered to the surface of the flask after one day observation. • The medium was changed every three days thereafter until the day seven, when cells showed ‘typical’ mesothelial cell morphology, and formed a monolayer and covered the surface of the flask completely.

Conclusion

• In conclusion, we suggest that the primary pleural mesothelial cell cultures, which were obtained in a laboratory set up in Turkey for the first time, may be used in experimental studies for pleural diseases.