Transcript Grids Cleaning_ A very important step for a successful data collection from

Grids Cleaning_
A very important step for a
successful data collection from
cryo electron microscopy
Zongli Li
Oct. 11, 2004
Why need to clean grid before use?
•
Good for your specimen
•
Good for imaging
•
Good for microscope
How to Clean grid?
Two organic solvents have been used in
this lab,
• Acetone ((CH3)2CO )
• ethyl acetate (CH3COOC2H5 )
People in other places also use
chloroform (CHCl3)
Diagram for cleaning grids-
Home-made holey grid (1)
Diagram for cleaning grids-
Home-made holey grid (2)
Quantifoil grid
In the final stage of the production process
of QUANTIFOIL® holey film, carbon is
evaporated onto a plastic holey film, and
the plastic is dissolved. The grids are
washed with chloroform, ethyl acetate and
acetone. However, not all plastic can be
removed. Therefore, the final thickness of
the foil is about 20 nm, although only 10
nm of carbon is evaporated onto the
plastic.
R1.2/1.3 quantifoil grid
Diagram for cleaning gridsQuantifoil grid
Acetone
water
water