Transcript OH EE Slawson Tempel, © WUSTL OH EE Slawson Tempel, © WUSTL.

OH
EE Slawson Tempel, © WUSTL
OH
EE Slawson Tempel, © WUSTL
OH
EE Slawson Tempel, © WUSTL
OH
OH
EE Slawson Tempel, © WUSTL
OH
OH
EE Slawson Tempel, © WUSTL
OH
OH
OH
OH
OH
EE Slawson Tempel, © WUSTL
OH
OH
EE Slawson Tempel, © WUSTL
OH
OH
OH
OH
OH
EE Slawson Tempel, © WUSTL
OH
OH
OH
EE Slawson Tempel, © WUSTL
OH
OH
OH
OH
OH
EE Slawson Tempel, © WUSTL
OH
OH
EE Slawson Tempel, © WUSTL
OH
EE Slawson Tempel, © WUSTL
3 2 1
EE Slawson Tempel, © WUSTL
OH
OH
EE Slawson Tempel, © WUSTL
OH
EE Slawson Tempel, © WUSTL
Pyrophosphate
OH
EE Slawson Tempel, © WUSTL
OH
EE Slawson Tempel, © WUSTL
ATP + luciferin
OH
EE Slawson Tempel, © WUSTL
ATP + luciferin
OH
EE Slawson Tempel, © WUSTL
OH
EE Slawson Tempel, © WUSTL
OH
OH
OH
OH
OH
EE Slawson Tempel, © WUSTL
OH
OH
OH
OH
EE Slawson Tempel, © WUSTL
OH
OH
OH
OH
EE Slawson Tempel, © WUSTL
OH
OH
OH
EE Slawson Tempel, © WUSTL
OH
OH
OH
EE Slawson Tempel, © WUSTL
OH
EE Slawson Tempel, © WUSTL
OH
EE Slawson Tempel, © WUSTL
ATP + luciferin
ATP + luciferin
ATP + luciferin
OH
EE Slawson Tempel, © WUSTL
ATP + luciferin
ATP + luciferin
ATP + luciferin
OH
EE Slawson Tempel, © WUSTL
Brightness of flash is proportional to number of nucleotides added
Flash brightness
4-mer
3-mer
2-mer
1mer
T…
EE Slawson Tempel, © WUSTL
Brightness of flash is proportional to number of nucleotides added
Flash brightness
4-mer
3-mer
2-mer
1mer
TC…
EE Slawson Tempel, © WUSTL
Brightness of flash is proportional to number of nucleotides added
Flash brightness
4-mer
3-mer
2-mer
1mer
TCA…
EE Slawson Tempel, © WUSTL
Brightness of flash is proportional to number of nucleotides added
Flash brightness
4-mer
3-mer
2-mer
1mer
TCAC…
EE Slawson Tempel, © WUSTL
Brightness of flash is proportional to number of nucleotides added
Flash brightness
4-mer
3-mer
2-mer
1mer
TCACTT…
EE Slawson Tempel, © WUSTL
Brightness of flash is proportional to number of nucleotides added
Flash brightness
4-mer
3-mer
2-mer
1mer
TCACTTC…
EE Slawson Tempel, © WUSTL
Brightness of flash is proportional to number of nucleotides added
Flash brightness
4-mer
3-mer
2-mer
1mer
TCACTTCAA…
EE Slawson Tempel, © WUSTL
Brightness of flash is proportional to number of nucleotides added
Flash brightness
4-mer
3-mer
2-mer
1mer
TCACTTCAAGGG…
EE Slawson Tempel, © WUSTL
Brightness of flash is proportional to number of nucleotides added
Flash brightness
4-mer
3-mer
2-mer
1mer
TCACTTCAAGGGT…
EE Slawson Tempel, © WUSTL
Brightness of flash is proportional to number of nucleotides added
Flash is
too bright
Flash brightness
4-mer
3-mer
2-mer
1mer
TCACTTCAAGGGT…
EE Slawson Tempel, © WUSTL
A
EE Slawson Tempel, © WUSTL
T
G
C
200 cycles
A
EE Slawson Tempel, © WUSTL
T
G
C
Read length
350-400 bp
200 cycles
A
EE Slawson Tempel, © WUSTL
T
G
C