Transcript EE Slawson Tempel, © WUSTL STOP STOP STOP STOP EE Slawson Tempel, © WUSTL.
EE Slawson Tempel, © WUSTL
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EE Slawson Tempel, © WUSTL
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EE Slawson Tempel, © WUSTL
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OH
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EE Slawson Tempel, © WUSTL
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EE Slawson Tempel, © WUSTL OH
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EE Slawson Tempel, © WUSTL
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EE Slawson Tempel, © WUSTL
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EE Slawson Tempel, © WUSTL
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EE Slawson Tempel, © WUSTL
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EE Slawson Tempel, © WUSTL
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OH
STOP STOP
OH
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EE Slawson Tempel, © WUSTL
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EE Slawson Tempel, © WUSTL OH
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EE Slawson Tempel, © WUSTL
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EE Slawson Tempel, © WUSTL
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EE Slawson Tempel, © WUSTL
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EE Slawson Tempel, © WUSTL
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EE Slawson Tempel, © WUSTL
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© Illumina, EEST, © WUSTL G …
© Illumina, EEST, © WUSTL G C …
© Illumina, EEST, © WUSTL G C T …
© Illumina, EEST, © WUSTL G C T G …
© Illumina, EEST, © WUSTL G C T G A …
Camera time is the limiting step!
Flowcell 8 lanes For picture taking: Each lane is broken up into 100 tiles, each fluor is imaged separately – 2400 pictures taken per cycle EE Slawson Tempel, © WUSTL
Chemistry problem 1: terminator is retained EE Slawson Tempel, © WUSTL
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Chemistry problem 2: fluor is retained EE Slawson Tempel, © WUSTL OH
Chemistry problem 2: fluor is retained EE Slawson Tempel, © WUSTL
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Chemistry problem 2: fluor is retained EE Slawson Tempel, © WUSTL
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