Chapter 6 Metabolism: Energy and Enzymes Copyright © The McGraw-Hill Companies, Inc.

Transcript Chapter 6 Metabolism: Energy and Enzymes Copyright © The McGraw-Hill Companies, Inc.

Chapter 6 Metabolism: Energy and Enzymes

Cells and the Flow of Energy •

Energy

is the ability to do work.

• Living things need to acquire energy; this is a characteristic of life.

• Cells use acquired energy to: • Maintain their organization • Carry out reactions that allow cells to develop, grow, and reproduce

Flow of energy

Cells and Entropy

• The term

entropy

is used to indicate the

relative state of disorganization

• Cells need a constant supply of energy to maintain their internal organization.

• Complex molecules like glucose tend to break apart into their building blocks, in this case carbon dioxide and water.

• This is because glucose is more organized, and thus less stable, than its breakdown products.

• The result is a loss of potential energy and an increase in entropy.

Metabolic Reactions and Energy Transformations •

Metabolism

is the sum of all the chemical reactions that occur in a cell.

•

Reactants

are substances that participate in a reaction;

products

are substances that form as a result of a reaction.

• A reaction will occur spontaneously if it increases entropy.

• Biologists use the term “

free energy

” instead of entropy for cells.

ATP: Energy for Cells • ATP

(

adenosine triphosphate

currency of cells. ) is the energy • ATP is constantly regenerated from

ADP

(

adenosine diphosphate

) after energy is expended by the cell.

• Use of ATP by the cell has advantages: • 1) It can be used in many types of reactions.

• 2) When ATP → ADP + P, energy released is sufficient for cellular needs and little energy is wasted.

The ATP cycle

Photosynthesis

• The overall reaction for photosynthesis can be written: •

6CO

+ 6H

O + energy → C

H

O

+ 6O

• During photosynthesis, hydrogen atoms are transferred from water to carbon dioxide, and glucose is formed.

2 • Water has been oxidized; carbon dioxide has been reduced.

• Energy to form glucose comes from the sun.

Cellular Respiration •

• The overall equation for cellular respiration is opposite that of photosynthesis:

C

H

Energy O

+ 6O

→ 6CO

+ 6H

O +

• In this reaction, glucose is oxidized and oxygen is reduced to become water.

• The complete oxidation of a mol of glucose releases

686 kcal

of energy that is used to synthesize ATP.

Organelles and the Flow of Energy

• During photosynthesis,

chloroplasts

capture solar energy and use it to convert water and carbon dioxide into carbohydrates that provide food for other living things.

• Cellular respiration, the breakdown of glucose into carbon dioxide and water, occurs in

mitochondria

• It is the cycling of molecules between chloroplasts and mitochondria that allows a flow of energy from the sun through all living things.

Relationship of chloroplasts to mitochondria

Chapter 7: Cellular Respiration

Cellular respiration

Cellular Respiration takes place in 4 phases Lets follow ONE molecule of glucose through its complete metabolism

Cellular Respiration takes place in 4 phases 1. Glycolysis is the breakdown of glucose into pyruvate, 2 ATP molecules are made Step 1: 2

Cellular Respiration takes place in 4 phases 2. In the transition reaction, pyruvate is broken down into acetyl CoA, no ATP is made Step 2: 2

Cellular Respiration takes place in 4 phases 3. The Citric Acid Cycle also called the “Krebs” cycle, and breaksdown Acetyl CoA into CO2….2 ATP are made Step 3: 2 MITOCHONDRIA 2

Cellular Respiration takes place in 4 phases 4. The Electron Transport System uses the ELECTRONS removed from glucose molecules to provide engergy to make TONS of ATP…..about 32-34 ATPs!!! Step 4: 2 MITOCHONDRIA 2 32

Cellular Respiration takes place in 4 phases Therefore, ONE molecule of glucose generates 2+2+32/34 ATP molecules…….a total of 36-38! Step 1: Step 2: Step 3: Step 4: 2 2 32

Where is all this occuring?!?!

Cell Outside of the Mitochondria!

ATP is not only produced….but also NADH and FADH!

ATP is the currency to run machinery within the cell…..

But NADH and FADH2 *which then makes the ATP!* run the electron transport system

NAD

and FAD

• Each step of cellular respiration requires a separate enzyme.

• Some enzymes use the oxidation reduction

coenzyme NAD +

(nicotinamide adenine dinucleotide).

•

FAD

(flavin adenine dinucleotide) is sometimes used instead of NAD + .

The function of NADH and FADH2 is to carry and then donate electrons to the electron transport system…..

*remember electron transport is occuring across the mitochondria’s cristae!*

What happens when breakdown of glucose is incomplete?

FERMENTATION!!!!

• When oxygen is available, pyruvate enters the

mitochondria

, where it undergoes further breakdown, through the citric acid and electron transport cycles.

• If oxygen is not available,

fermentation

pyruvate undergoes

reduction

occurs and • Fermentation is an

anaeorbic

process and does not require oxygen. (“an-aeorobic" means, without oxygen) • In humans, pyruvate is reduced to

lactic acid

fermentation.

during

In humans…..

In bacteria or yeast…..

The Fermentation Process…… remember, it’s ANAEOROBIC this situation only occurs when oxygen levels are LOW!

In humans…..

In bacteria or yeast…..

Notice, that in low oxygen you only make 2 ATP, compared to 36!

this is why, when you have an oxygen debt, you get a lactic acid buildup in your muscles! AND, you pant to try to Bring more oxygen into your body to complete cellular respiration

Efficiency of Fermentation

• Two ATP produced during fermentation are equivalent to

14.6 kcal

; complete oxidation of glucose to CO 2 yield of

686 kcal

and H 2 O represents a per molecule of glucose.

• Thus, fermentation is only

2.1% efficient

compared to cellular respiration.

• (14.6/686) x 100 = 2.1%

Advantages and Disadvantages of Fermentation •

• Fermentation can provide a rapid burst of ATP in muscle cells, even when oxygen is in limited supply.

• Lactate, however, is toxic to cells.

• Initially, blood carries away lactate as it forms; eventually lactate builds up, lowering cell pH, and causing muscles to fatigue.

Oxygen debt

occurs, and the liver must reconvert lactate to pyruvate.

But we don’t just eat carbohydrates…..so what happens with proteins and lipids?

? ?

The metabolic pool concept

Catabolism

catabolic reactions, break it down • Molecules aside from glucose can enter the catabolic reactions of cellular respiration.

• When a fat is used for energy, it breaks down into

glycerol

and three

fatty acids

; glycerol is converted to PGAL, and the fatty acids are converted to acetyl-CoA, thus both types of molecules can enter the citric acid cycle.

Fat

breaks down into

glycerol

and three

fatty acids

; glycerol is converted to PGAL, and the fatty acids are converted to acetyl-CoA, and both molecules can then enter the citric acid cycle.

Catabolism

catabolic reactions, break it down • The carbon backbones of

amino acids

also enter the reactions of cellular respiration to provide energy.

• Where the carbon portion of the amino acid enters the reactions of respiration depends on its number of carbons. can • The amino acid first undergoes

deamination

, or the removal of the amino group in the liver; the amino group becomes ammonia (NH3) and is excreted as urea.

Anabolism

Anabolic reactions build things up • The substrates of the pathways of cellular respiration can also be used as starting materials for synthetic reactions.

• This is the cell’s

metabolic pool

, in which one type of molecule can be converted into another.

• In this way,

dietary carbohydrates can be converted to stored fat

, and come substrates of the citric acid cycle can be

transaminated

into amino acids.

Phases of Complete Glucose Breakdown • •

• The oxidation of glucose by removal of hydrogen atoms involves four phases:

Glycolysis

– the breakdown of glucose to two molecules of

pyruvate

in the cytoplasm with no oxygen needed; yields 2 ATP

Transition reaction

– pyruvate is oxidized to a 2-carbon acetyl group carried by CoA, and CO 2 is removed; occurs twice per glucose molecule

• •

Citric acid cycle

– a cyclical series of oxidation reactions that give off CO 2 and produce one ATP per cycle; occurs twice per glucose molecule

Electron transport system

– a series of carriers that accept electrons removed from glucose and pass them from one carrier to the next until the final receptor, O 34 ATP 2 is reached; water is produced; energy is released and used to synthesize 32 to • If oxygen is not available, fermentation occurs in the cytoplasm instead of proceeding to cellular respiration.

Chapter 6 Metabolism: Energy and Enzymes Copyright © The McGraw-Hill Companies, Inc.

Transcript Chapter 6 Metabolism: Energy and Enzymes Copyright © The McGraw-Hill Companies, Inc.