Transcript Harvard iGEM 2006 Week 3 Peng, David, Jeff, Hetmann E. Coli  We aim to create a kaiABC + reporter BioBrick which expresses a circadian.

Harvard iGEM 2006
Week 3
Peng, David, Jeff, Hetmann
E. Coli

We aim to create a kaiABC + reporter BioBrick which
expresses a circadian rhythm in both E. coli and
cyanobacteria.

(We are still brainstorming ideas of what to do; this
week was review literature / grow strains / contact
people)
Blue = biobricks cut site
Green = can be obtained from
cyanobacteria
Prof. Susan Golden and
others have this
We’ve obtained three strains of cyanobacteria:
 From Eric Webb at Woods Hole: WH8102, a marine strain
 We’re probably not going to use it because it’s reputed to be very difficult to
grow and modify.
 From Peter Weigele at MIT: PCC7942 and PCC6803, both
freshwater strains
 PCC6803 can metabolize glucose.
 PCC7942 is the most common strain we’ve encountered in the literature on
cyanobacteria circadian oscillation.
 Both strains’ genomes have been sequenced.
 Both can be reliably transformed by exogenous DNA.
 We modified an incubator by installing a timer-controlled
fluorescent light (32W + 22W bulbs)
 The lights are set to stay on for 16 hours each day
 According to Peter Weigele, we should expect growth in 5-7 days
(Tuesday-Thursday this week)
 Design primers for extracting KaiABC
 Learn protocols for transforming PCC7942 and
PCC6803
 (Maybe): synthesis of kaiABC
 Waiting to hear back from…
 Susan Golden at UT Austin
 Alexander van Oodenaarden at MIT
 Jeffrey Chabot